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PMID: 9826638 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Temperature transitions of protein properties in human red blood cells.

Biophysical journal ·Vol. 75 ·No. 6 ·1998-12-00 ·Pages 3179-83

Artmann GM, Kelemen C, Porst D, Büldt G, Chien S

Abstract

Human red blood cells (RBC) undergo a sudden change from blocking to passing through 1.3 +/- 0.2-micrometer micropipettes at a transition temperature (Tc) of 36.4 degrees C. For resealed RBC ghosts this transition occurs at 28.3 degrees C (Tg). These findings are attributed to an elastomeric transition of hemoglobin from being gel-like to a fluid and to an elastomeric transition of membrane proteins such as spectrin. Spectrin shows a uniform distribution along the aspirated RBC tongue above Tg in contrast to the linear gradient below Tg.

MeSH Terms
Biophysical Phenomena Biophysics Blood Proteins/chemistry,physiology Elasticity Erythrocyte Deformability/physiology Erythrocyte Membrane/chemistry,physiology Erythrocytes/chemistry,physiology Gels Hemoglobins/chemistry,physiology Humans In Vitro Techniques Spectrin/chemistry,physiology Temperature Thermodynamics Viscosity
Chemicals
Blood Proteins Gels Hemoglobins Spectrin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Artmann G M
Department of Cell Biophysics, Aachen University of Applied Sciences, Ginsterweg 1, D-52428 Jülich, Germany. [email protected]
Kelemen C
Porst D
Büldt G
Chien S
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(98)77759-8
Email
[email protected]
Published
1998-12-00
Pages
3179-83
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1299989
Subset
IM
Grants
NHLBI NIH HHS · HL 43026 · United States
NHLBI NIH HHS · HL 44147 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(98)77759-8 PMC Full Text View journal details
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