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PMID: 9832537 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Retrotransposon-related DNA sequences in the centromeres of grass chromosomes.

Genetics ·Vol. 150 ·No. 4 ·1998-12-00 ·Pages 1615-23

Miller JT, Dong F, Jackson SA, Song J, Jiang J

Abstract

Several distinct DNA fragments were subcloned from a sorghum (Sorghum bicolor) bacterial artificial chromosome clone 13I16 that was derived from a centromere. Three fragments showed significant sequence identity to either Ty3/gypsy- or Ty1/copia-like retrotransposons. Fluorescence in situ hybridization (FISH) analysis revealed that the Ty1/copia-related DNA sequences are not specific to the centromeric regions. However, the Ty3/gypsy-related sequences were present exclusively in the centromeres of all sorghum chromosomes. FISH and gel-blot hybridization showed that these sequences are also conserved in the centromeric regions of all species within Gramineae. Thus, we report a new retrotransposon that is conserved in specific chromosomal regions of distantly related eukaryotic species. We propose that the Ty3/gypsy-like retrotransposons in the grass centromeres may be ancient insertions and are likely to have been amplified during centromere evolution. The possible role of centromeric retrotransposons in plant centromere function is discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Centromere Conserved Sequence DNA, Plant Molecular Sequence Data Poaceae/genetics Retroelements
Chemicals
DNA, Plant Retroelements
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Miller J T
Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, USA.
Dong F
Jackson S A
Song J
Jiang J
References (30)
30 references, click to expand
  1. The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 has unusual regulatory elements that direct high-level expression.
    Plant Physiol. 1996 Oct;112(2):727-33 PMID: 8883384
  2. Comparative analysis of the chromosomal and genomic organization of Ty1-copia-like retrotransposons in pteridophytes, gymnosperms and angiosperms.
    Plant Mol Biol. 1997 Jan;33(1):11-21 PMID: 9037155
  3. The unusual telomeres of Drosophila.
    Trends Genet. 1995 Feb;11(2):58-62 PMID: 7716808
  4. Centromeres of budding and fission yeasts.
    Trends Genet. 1990 May;6(5):150-4 PMID: 2195725
  5. Sequence analysis of closely related retrotransposon families from fission yeast.
    Gene. 1993 Sep 6;131(1):135-9 PMID: 8396547
  6. Undermethylation associated with retroelement activation and chromosome remodelling in an interspecific mammalian hybrid.
    Nature. 1998 May 7;393(6680):68-72 PMID: 9590690
  7. DNA regions flanking the major Arabidopsis thaliana satellite are principally enriched in Athila retroelement sequences.
    Genetica. 1996 Mar;97(2):141-51 PMID: 8984010
  8. The distribution of transposable elements within and between chromosomes in a population of Drosophila melanogaster. III. Element abundances in heterochromatin.
    Genet Res. 1994 Dec;64(3):183-97 PMID: 7698642
  9. Duplication of a gene-rich cluster between 16p11.1 and Xq28: a novel pericentromeric-directed mechanism for paralogous genome evolution.
    Hum Mol Genet. 1996 Jul;5(7):899-912 PMID: 8817324
  10. Rice (Oryza sativa) centromeric regions consist of complex DNA.
    Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8135-40 PMID: 9653153
  11. Origin and evolution of retroelements based upon their reverse transcriptase sequences.
    EMBO J. 1990 Oct;9(10):3353-62 PMID: 1698615
  12. Do Plants Have a One-Way Ticket to Genomic Obesity?
    Plant Cell. 1997 Sep;9(9):1509-1514 PMID: 12237393
  13. Molecular structure of a functional Drosophila centromere.
    Cell. 1997 Dec 26;91(7):1007-19 PMID: 9428523
  14. Centromeres, CENP-B and Tigger too.
    Trends Genet. 1997 Apr;13(4):141-5 PMID: 9097724
  15. LTR-retrotransposons and MITEs: important players in the evolution of plant genomes.
    Curr Opin Genet Dev. 1995 Dec;5(6):814-21 PMID: 8745082
  16. CENP-B binds a novel centromeric sequence in the Asian mouse Mus caroli.
    Mol Cell Biol. 1995 Aug;15(8):4009-20 PMID: 7623797
  17. Centromeres of mammalian chromosomes.
    Trends Genet. 1990 Dec;6(12):410-6 PMID: 2087784
  18. A conserved repetitive DNA element located in the centromeres of cereal chromosomes.
    Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):14210-3 PMID: 8943086
  19. Localization of centromere function in a Drosophila minichromosome.
    Cell. 1995 Aug 25;82(4):599-609 PMID: 7664339
  20. Construction of a rice bacterial artificial chromosome library and identification of clones linked to the Xa-21 disease resistance locus.
    Plant J. 1995 Mar;7(3):525-33 PMID: 7757120
  21. Interchromosomal duplications of the adrenoleukodystrophy locus: a phenomenon of pericentromeric plasticity.
    Hum Mol Genet. 1997 Jul;6(7):991-1002 PMID: 9215666
  22. Complete nucleotide sequence of the Drosophila transposable element copia: homology between copia and retroviral proteins.
    Mol Cell Biol. 1985 Jul;5(7):1630-8 PMID: 2410772
  23. Misdivision analysis of centromere structure in maize.
    EMBO J. 1996 Oct 1;15(19):5246-55 PMID: 8895570
  24. The centromeric K-type repeat and the central core are together sufficient to establish a functional Schizosaccharomyces pombe centromere.
    Mol Biol Cell. 1994 Jul;5(7):747-61 PMID: 7812044
  25. Date of the monocot-dicot divergence estimated from chloroplast DNA sequence data.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6201-5 PMID: 2762323
  26. Nested retrotransposons in the intergenic regions of the maize genome.
    Science. 1996 Nov 1;274(5288):765-8 PMID: 8864112
  27. Emergence and scattering of multiple neurofibromatosis (NF1)-related sequences during hominoid evolution suggest a process of pericentromeric interchromosomal transposition.
    Hum Mol Genet. 1997 Jan;6(1):9-16 PMID: 9002664
  28. BIS 1, a major component of the cereal genome and a tool for studying genomic organization.
    Genomics. 1991 Jun;10(2):469-76 PMID: 2071151
  29. Interphase fluorescence in situ hybridization mapping: a physical mapping strategy for plant species with large complex genomes.
    Mol Gen Genet. 1996 Oct 16;252(5):497-502 PMID: 8914510
  30. The contributions of retroelements to plant genome organization, function and evolution.
    Trends Microbiol. 1996 Sep;4(9):347-53 PMID: 8885169
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/150.4.1615
Published
1998-12-00
Pages
1615-23
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460426
Subset
IM
Databases
GENBANK
AF078901, AF078903
External Links
PubMed source DOI: 10.1093/genetics/150.4.1615 PMC Full Text View journal details
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