Changes in bacterial species composition in enrichment cultures with various dilutions of inoculum as monitored by denaturing gradient gel electrophoresis.

Applied and environmental microbiology ·Vol. 64 ·No. 12 ·1998-12-00 ·Pages 5046-8

Jackson CR, Roden EE, Churchill PF

Abstract

Denaturing gradient gel electrophoresis revealed changes in the bacterial species obtained from enrichment cultures with different inoculum dilutions. This inoculum dilution enrichment approach may facilitate the detection and isolation of a greater number of bacterial species than traditional enrichment techniques.

MeSH Terms

Bacteria/classification,genetics,isolation & purification Biofilms DNA, Ribosomal/genetics Fresh Water Molecular Sequence Data Plant Leaves Plants/microbiology RNA, Ribosomal, 16S/genetics,isolation & purification Water Microbiology

Chemicals

DNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jackson C R

Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama 35487, USA. [email protected]

Roden E E

Churchill P F

References (9)

9 references, click to expand

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Appl Environ Microbiol. 1996 Nov;62(11):3922-8 PMID: 8899977
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Appl Environ Microbiol. 1997 Mar;63(3):983-9 PMID: 9055415
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.64.12.5046-5048.1998

[email protected]

Published

1998-12-00

Pages

5046-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC90967

Subset

Databases

GENBANK

AF061035, AF061036

External Links

PubMed source DOI: 10.1128/AEM.64.12.5046-5048.1998 PMC Full Text View journal details

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Changes in bacterial species composition in enrichment cultures with various dilutions of inoculum as monitored by denaturing gradient gel electrophoresis.

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