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PMID: 9844048 Published · ppublish English Journal Article

Differential regulation of Th1 and Th2 cells by p91-110 and p21-40 peptides of the 16-kD alpha-crystallin antigen of Mycobacterium tuberculosis.

Clinical and experimental immunology ·Vol. 114 ·No. 3 ·1998-12-00 ·Pages 392-7

Agrewala JN, Wilkinson RJ

Abstract

Permissively recognized peptides which can activate lymphocytes from subjects with a variety of class II HLA types are interesting diagnostic and vaccine candidates. In this study we generated T helper clones reactive to the permissively recognized p21-40 and p91-110 peptides of the 16-kD heat shock protein of Mycobacterium tuberculosis. All the clones specific for p91-110 secreted interferon-gamma (IFN-gamma) and were of the Th1 phenotype. By contrast, the p21-40 peptide favoured the generation of IL-4-producing clones. Antibody blockade established that the peptide-specific Th clones could either be DR-, DP- or DQ-restricted. Thus, two permissively recognized sequences p21-40 and p91-110 from the same mycobacterial antigen can drive the differentiation of functionally distinct T helper subsets. Attempts to immunize against tuberculosis should bear in mind epitope specificity if a favourable Th subtype response is to be generated.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/immunology Cell Line Cytokines Dose-Response Relationship, Drug HLA-DP Antigens/immunology HLA-DQ Antigens/immunology HLA-DR Antigens/immunology Humans Immunophenotyping Molecular Sequence Data Mycobacterium tuberculosis/immunology Peptide Fragments/immunology Th1 Cells/immunology Th2 Cells/immunology
Chemicals
Antigens, Bacterial Cytokines HLA-DP Antigens HLA-DQ Antigens HLA-DR Antigens Peptide Fragments Mycobacterium tuberculosis antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Agrewala J N
Tuberculosis & Related Infections Unit, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
Wilkinson R J
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
DOI
10.1046/j.1365-2249.1998.00724.x
Published
1998-12-00
Pages
392-7
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1905128
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1046/j.1365-2249.1998.00724.x PMC Full Text View journal details
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