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PMID: 9851933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Eight calves cloned from somatic cells of a single adult.

Science (New York, N.Y.) ·Vol. 282 ·No. 5396 ·1998-12-11 ·Pages 2095-8

Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y

Abstract

Eight calves were derived from differentiated cells of a single adult cow, five from cumulus cells and three from oviductal cells out of 10 embryos transferred to surrogate cows (80 percent success). All calves were visibly normal, but four died at or soon after birth from environmental causes, and postmortem analysis revealed no abnormality. These results show that bovine cumulus and oviductal epithelial cells of the adult have the genetic content to direct the development of newborn calves.

MeSH Terms

Animal Husbandry Animals Blastocyst/cytology Cattle/embryology,genetics Cell Fusion Cells, Cultured Cloning, Organism/methods Cytoplasm/physiology Embryo Transfer/veterinary Epithelial Cells/cytology Fallopian Tubes/cytology Female Microsatellite Repeats Nuclear Transfer Techniques Oocytes/cytology Ovarian Follicle/cytology Pregnancy Pregnancy, Multiple

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Kato Y

Laboratory of Animal Reproduction, College of Agriculture and Research Institute for Animal Developmental Biotechnology, Kinki University, 3327-204, Nakamachi, Nara, 631-8505, Japan.

Tani T

Sotomaru Y

Kurokawa K

Kato J

Doguchi H

Yasue H

Tsunoda Y

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.282.5396.2095

Published

1998-12-11

Pages

2095-8

Language

English

Region

United States

NLM ID

0404511

Subset

Corrections

CommentIn

PMID: 9874644

External Links

PubMed source DOI: 10.1126/science.282.5396.2095 View journal details

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Eight calves cloned from somatic cells of a single adult.

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