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PMID: 9851982 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

BMP4 is essential for lens induction in the mouse embryo.

Genes & development ·Vol. 12 ·No. 23 ·1998-12-01 ·Pages 3764-75

Furuta Y, Hogan BL

Abstract

Vertebrate lens development is a classical model system for studying embryonic tissue interactions. Little is known, however, about the molecules mediating such inductive events. Here, we show that Bmp4, which is expressed strongly in the optic vesicle and weakly in the surrounding mesenchyme and surface ectoderm, has crucial roles during lens induction. In Bmp4(tm1) homozygous null mutant embryos, lens induction is absent, but the process can be rescued by exogenous BMP4 protein applied into the optic vesicle in explant cultures. This is associated with rescue of ectodermal expression of Sox2, an early lens placode marker. Substituting the optic vesicle in explant cultures with BMP4-carrying beads, however, does not lead to lens induction, indicating that other factors produced by the optic vesicle are involved. BMP4 appears to regulate expression of a putative downstream gene, Msx2, in the optic vesicle. No change in Pax6 expression is seen in Bmp4(tm1) mutant eyes, and Bmp4 expression appears unaffected in the eyes of homozygous Pax6(Sey-1Neu), suggesting that PAX6 and BMP4 function independently. Based on these results we propose that BMP4 is required for the optic vesicle to manifest its lens-inducing activity, by regulating downstream genes and/or serving as one component of multiple inductive signals.

MeSH Terms
Animals Antigens, Differentiation Bone Morphogenetic Protein 4 Bone Morphogenetic Protein Receptors Bone Morphogenetic Proteins/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Ectoderm Embryonic Induction Eye/embryology Eye Proteins Gene Expression Regulation, Developmental Homeodomain Proteins Homozygote In Vitro Techniques Lens, Crystalline/abnormalities,embryology Mesoderm Mice Mice, Mutant Strains Models, Biological Nervous System/embryology PAX6 Transcription Factor Paired Box Transcription Factors Receptors, Cell Surface/metabolism Receptors, Growth Factor Repressor Proteins Tissue Transplantation
Chemicals
Antigens, Differentiation Bmp4 protein, mouse Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins DNA-Binding Proteins Eye Proteins Homeodomain Proteins PAX6 Transcription Factor Paired Box Transcription Factors Pax6 protein, mouse Receptors, Cell Surface Receptors, Growth Factor Repressor Proteins Bone Morphogenetic Protein Receptors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Furuta Y
Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232-2175 USA.
Hogan B L
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.23.3764
Published
1998-12-01
Pages
3764-75
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317259
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.23.3764 PMC Full Text View journal details
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