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PMID: 9852584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synaptic modifications in cultured hippocampal neurons: dependence on spike timing, synaptic strength, and postsynaptic cell type.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 24 ·1998-12-15 ·Pages 10464-72

Bi GQ, Poo MM

Abstract

In cultures of dissociated rat hippocampal neurons, persistent potentiation and depression of glutamatergic synapses were induced by correlated spiking of presynaptic and postsynaptic neurons. The relative timing between the presynaptic and postsynaptic spiking determined the direction and the extent of synaptic changes. Repetitive postsynaptic spiking within a time window of 20 msec after presynaptic activation resulted in long-term potentiation (LTP), whereas postsynaptic spiking within a window of 20 msec before the repetitive presynaptic activation led to long-term depression (LTD). Significant LTP occurred only at synapses with relatively low initial strength, whereas the extent of LTD did not show obvious dependence on the initial synaptic strength. Both LTP and LTD depended on the activation of NMDA receptors and were absent in cases in which the postsynaptic neurons were GABAergic in nature. Blockade of L-type calcium channels with nimodipine abolished the induction of LTD and reduced the extent of LTP. These results underscore the importance of precise spike timing, synaptic strength, and postsynaptic cell type in the activity-induced modification of central synapses and suggest that Hebb's rule may need to incorporate a quantitative consideration of spike timing that reflects the narrow and asymmetric window for the induction of synaptic modification.

MeSH Terms
6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology Action Potentials/drug effects,physiology Animals Bicuculline/pharmacology Calcium Channels/drug effects,physiology Cells, Cultured Embryo, Mammalian Excitatory Postsynaptic Potentials/drug effects,physiology Glutamic Acid/physiology Hippocampus/physiology In Vitro Techniques Long-Term Potentiation Neurons/drug effects,physiology Nimodipine/pharmacology Patch-Clamp Techniques Rats Synapses/drug effects,physiology Time Factors gamma-Aminobutyric Acid/physiology
Chemicals
Calcium Channels Glutamic Acid gamma-Aminobutyric Acid Nimodipine 6-Cyano-7-nitroquinoxaline-2,3-dione Bicuculline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bi G Q
Department of Biology, University of California at San Diego, La Jolla, California 92093, USA.
Poo M M
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-12-15
Pages
10464-72
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793365
Subset
IM
Grants
NINDS NIH HHS · R01 NS036999 · United States
NINDS NIH HHS · NS36999 · United States
External Links
PubMed source PMC Full Text View journal details
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