Cholinergically stimulated phosphatidylinositol breakdown in parotid-gland fragments is independent of the ionic environment.

The Biochemical journal ·Vol. 158 ·No. 2 ·1976-08-15 ·Pages 505-7

Jones LM, Michell RH

Abstract

Carbamoylcholine stimulated phosphatidylinositol breakdown in rat parotid-gland fragments incubated in either a Krebs--Ringer medium or an ion-depleted iso-osmotic sucrose medium. This suggest that phosphatidylinositol breakdown is not initiated by a change in the intracellular concentration of Na+ or of K+, and previous experiments have already indicated that it is independent of cyclic nucleotides and Ca2+. Thus it seems that this reaction may be initiated by a direct interaction at the plasma membrane between the activated muscarinic cholinergic receptor system and the enzyme that catalyses phosphatidylinositol breakdown.

MeSH Terms

Animals Calcium/metabolism Carbachol/pharmacology Cell Membrane/metabolism Male Parotid Gland/metabolism Phosphatidylinositols/metabolism Potassium/metabolism Rats Sodium/metabolism

Chemicals

Phosphatidylinositols Carbachol Sodium Potassium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jones L M

Michell R H

References (12)

12 references, click to expand

Muscarinic cholinergic stimulation of phosphatidylinositol turnover in the longitudinal smooth muscle of guinea-pig ileum.
Biochem J. 1976 Mar 15;154(3):653-7 PMID: 182125
Stimulation of phosphatidylinositol turnover by histamine, 5-hydroxytryptamine and adrenaline in the longitudinal smooth muscle of guinea pig ileum.
Biochem Pharmacol. 1976 Jun 15;25(12):1429-30 PMID: 180997
Inositol phospholipids and cell surface receptor function.
Biochim Biophys Acta. 1975 Mar 25;415(1):81-47 PMID: 164246
Incorporation of inorganic [32P] phosphate into rat parotid phosphatidylinositol. Induction through activation of alpha adrenergic and cholinergic receptors and relation to K+ release.
Mol Pharmacol. 1975 Jan;11(1):79-86 PMID: 163953
Influence of potassium and calcium ions on the phosphatidylinositol and phosphatidylcholine metabolism in rat fibroblasts after growth stimulation by calf serum.
Eur J Biochem. 1975 Aug 15;56(2):413-20 PMID: 170106
Production of 1,2-diacylglycerol and phosphatidate in human erythrocytes treated with calcium ions and ionophore A23187.
Biochem J. 1976 May 15;156(2):225-32 PMID: 821476
Enhanced synthesis de novo of phosphatidylinositol in lymphocytes treated with cationic amphiphilic drugs.
Biochem J. 1975 Jun;148(3):471-8 PMID: 173283
The role of Na+ and K+ in the stimulation by thyrotropin of phospholipid turnover in bovine thyroid slices.
Endocrinology. 1974 Dec;95(6):1529-33 PMID: 4434900
Calcium and energy requirements for K + release mediated by the epinephrine -receptor in rat parotid slices.
J Biol Chem. 1973 Jan 10;248(1):369-72 PMID: 4692840
Breakdown of phosphatidylinositol provoked by muscarinic cholinergic stimulation of rat parotid-gland fragments.
Biochem J. 1974 Sep;142(3):583-90 PMID: 4377209
Enhanced phosphatidylinositol labelling in rat parotid fragments exposed to alpha-adrenergic stimulation.
Biochem J. 1974 Jan;138(1):47-52 PMID: 4365258
The relationship of calcium to receptor-controlled stimulation of phosphatidylinositol turnover. Effects of acetylcholine, adrenaline, calcium ions, cinchocaine and a bivalent cation ionophore on rat parotid-gland fragments.
Biochem J. 1975 Jun;148(3):479-85 PMID: 173284

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1580505

Published

1976-08-15

Pages

505-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1163998

Subset

External Links

PubMed source DOI: 10.1042/bj1580505 PMC Full Text View journal details

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Cholinergically stimulated phosphatidylinositol breakdown in parotid-gland fragments is independent of the ionic environment.

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