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PMID: 9858550 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA.

Molecular and cellular biology ·Vol. 19 ·No. 1 ·1999-01-00 ·Pages 261-73

Schaal TD, Maniatis T

Abstract

We have identified multiple distinct splicing enhancer elements within protein-coding sequences of the constitutively spliced human beta-globin pre-mRNA. Each of these highly conserved sequences is sufficient to activate the splicing of a heterologous enhancer-dependent pre-mRNA. One of these enhancers is activated by and binds to the SR protein SC35, whereas at least two others are activated by the SR protein SF2/ASF. A single base mutation within another enhancer element inactivates the enhancer but does not change the encoded amino acid. Thus, overlapping protein coding and RNA recognition elements may be coselected during evolution. These studies provide the first direct evidence that SR protein-specific splicing enhancers are located within the coding regions of constitutively spliced pre-mRNAs. We propose that these enhancers function as multisite splicing enhancers to specify 3' splice-site selection.

MeSH Terms
Cross-Linking Reagents Enhancer Elements, Genetic Exons Globins/genetics Humans Nuclear Proteins/genetics,metabolism RNA Precursors RNA Splicing RNA-Binding Proteins Ribonucleoproteins Serine-Arginine Splicing Factors
Chemicals
Cross-Linking Reagents Nuclear Proteins RNA Precursors RNA-Binding Proteins Ribonucleoproteins SRSF2 protein, human Serine-Arginine Splicing Factors Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schaal T D
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Maniatis T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.1.261
Published
1999-01-00
Pages
261-73
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC83884
Subset
IM
Grants
NIGMS NIH HHS · GM42231 · United States
External Links
PubMed source DOI: 10.1128/MCB.19.1.261 PMC Full Text View journal details
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