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PMID: 987581 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A general method for isolation of high molecular weight DNA from eukaryotes.

Nucleic acids research ·Vol. 3 ·No. 9 ·1976-09-00 ·Pages 2303-8

Blin N, Stafford DW

Abstract

A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.

MeSH Terms
Animals Cattle DNA/isolation & purification Dinoflagellida/analysis Female Humans Liver/analysis Methods Mice Molecular Weight Placenta/analysis Pregnancy Thymus Gland/analysis Ultracentrifugation
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blin N
Stafford D W
References (8)
8 references, click to expand
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  4. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  5. Sedimentation properties of yeast nuclear and mitochondrial DNA.
    J Mol Biol. 1972 Jun 14;67(1):11-24 PMID: 4557599
  6. Hydrodynamic shear breakage of DNA.
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  8. The nucleic acids of Drosophila melanogaster.
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.9.2303
Published
1976-09-00
Pages
2303-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343085
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.9.2303 PMC Full Text View journal details
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