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PMID: 9882344 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Coxsackievirus and adenovirus receptor amino-terminal immunoglobulin V-related domain binds adenovirus type 2 and fiber knob from adenovirus type 12.

Journal of virology ·Vol. 73 ·No. 2 ·1999-02-00 ·Pages 1392-8

Freimuth P, Springer K, Berard C, Hainfeld J, Bewley M, Flanagan J

Abstract

The extracellular region of the coxsackievirus and adenovirus receptor (CAR) is predicted to consist of two immunoglobulin (Ig)-related structural domains. We expressed the isolated CAR amino-terminal domain (D1) and a CAR fragment containing both extracellular Ig domains (D1/D2) in Escherichia coli. Both D1 and D1/D2 formed complexes in vitro with the recombinant knob domain of adenovirus type 12 (Ad12) fiber, and D1 inhibited adenovirus type 2 (Ad2) infection of HeLa cells. These results indicate that the adenovirus-binding activity of CAR is localized in the amino-terminal IgV-related domain and confirm our earlier observation that Ad2 and Ad12 bind to the same cellular receptor. Preliminary crystallization studies suggest that complexes of Ad12 knob bound to D1 will be suitable for structure determination.

MeSH Terms
Adenoviruses, Human/metabolism Binding Sites Capsid/metabolism Capsid Proteins Coxsackie and Adenovirus Receptor-Like Membrane Protein Enterovirus B, Human/metabolism Epitopes, B-Lymphocyte/genetics,immunology,metabolism Gene Expression HeLa Cells Humans Immunoglobulin Variable Region/immunology Receptors, Virus/genetics,immunology,metabolism
Chemicals
CLMP protein, human Capsid Proteins Coxsackie and Adenovirus Receptor-Like Membrane Protein Epitopes, B-Lymphocyte Immunoglobulin Variable Region Receptors, Virus hexon capsid protein, Adenovirus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Freimuth P
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA. [email protected]
Springer K
Berard C
Hainfeld J
Bewley M
Flanagan J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.2.1392-1398.1999
Email
[email protected]
Published
1999-02-00
Pages
1392-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC103963
Subset
IM
Grants
NIAID NIH HHS · R01 AI036251 · United States
NIAID NIH HHS · AI36251 · United States
External Links
PubMed source DOI: 10.1128/JVI.73.2.1392-1398.1999 PMC Full Text View journal details
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