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PMID: 9882664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CtaA of Staphylococcus aureus is required for starvation survival, recovery, and cytochrome biosynthesis.

Journal of bacteriology ·Vol. 181 ·No. 2 ·1999-01-00 ·Pages 501-7

Clements MO, Watson SP, Poole RK, Foster SJ

Abstract

A Staphylococcus aureus mutant (SPW3) apparently unable to survive long-term starvation was shown to have a transposon insertion within a gene homologous to ctaA of Bacillus subtilis which encodes a heme A synthase. Analysis of the cytochrome profiles of SPW3 revealed the absence of heme A-containing cytochromes compared to the parental 8325-4 strain. SPW3 demonstrated a 100-fold reduction in the ability to survive starvation induced by glucose limitation, under aerated conditions, compared to 8325-4. Analysis of starved cultures revealed that greater than 90% of the cells which demonstrated metabolism (as shown by rhodamine 123 accumulation) were unable to recover and form colonies on agar. Analysis of the lag phase and initial growth kinetics of those cells which could recover also showed a defect. This recovery defect could be partially alleviated by the inclusion of catalase in the recovery medium, indicating the probable involvement of oxidative stress. SPW3 also exhibited reduced colony size similar to that of a small-colony variant, increased resistance to aminoglycoside antibiotics, and reduced hemolysin and toxic shock syndrome toxin 1 production, but no alteration in the ability to form lesions in a subcutaneous mouse infection model.

MeSH Terms
Aerobiosis Amino Acid Sequence Animals Bacillus subtilis/genetics Bacterial Proteins/genetics,metabolism Catalase/metabolism Cloning, Molecular Culture Media Cytochrome b Group/biosynthesis,chemistry,genetics Cytochromes/biosynthesis DNA Transposable Elements Kinetics Membrane Proteins/biosynthesis,chemistry,genetics Mice Molecular Sequence Data Oxidative Stress Recombinant Proteins/biosynthesis,chemistry Sequence Alignment Sequence Homology, Amino Acid Staphylococcal Infections/microbiology Staphylococcus aureus/genetics,growth & development,physiology
Chemicals
Bacterial Proteins CtaA protein, bacteria Culture Media Cytochrome b Group Cytochromes DNA Transposable Elements Membrane Proteins Recombinant Proteins Catalase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clements M O
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Watson S P
Poole R K
Foster S J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.2.501-507.1999
Published
1999-01-00
Pages
501-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93404
Subset
IM
Databases
GENBANK
AF072726
External Links
PubMed source DOI: 10.1128/JB.181.2.501-507.1999 PMC Full Text View journal details
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