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PMID: 9885247 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamic localization of CLIP-170 to microtubule plus ends is coupled to microtubule assembly.

The Journal of cell biology ·Vol. 144 ·No. 1 ·1999-01-11 ·Pages 99-112

Diamantopoulos GS, Perez F, Goodson HV, Batelier G, Melki R, Kreis TE, Rickard JE

Abstract

CLIP-170 is a cytoplasmic linker protein that localizes to plus ends of microtubules in vivo. In this study, we have characterized the microtubule-binding properties of CLIP-170, to understand the mechanism of its plus end targeting. We show that the NH2-terminal microtubule-interacting domain of CLIP-170 alone localizes to microtubule plus ends when transfected into cells. Association of CLIP-170 with newly-formed microtubules was observed in cells microinjected with biotinylated tubulin, used as a tracer for growing microtubules. Using in vitro assays, association of CLIP-170 with recently polymerized tubulin is also seen. Cross-linking and sedimentation velocity experiments suggest association of CLIP-170 with nonpolymerized tubulin. We conclude from these experiments that the microtubule end targeting of CLIP-170 is closely linked to tubulin polymerization.

MeSH Terms
Centrosome/metabolism Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Guanosine Triphosphate/analogs & derivatives,metabolism HeLa Cells Humans Microtubule-Associated Proteins/metabolism Microtubules/metabolism,physiology Neoplasm Proteins Tubulin/metabolism Tumor Cells, Cultured
Chemicals
Microtubule-Associated Proteins Neoplasm Proteins Tubulin cytoplasmic linker protein 170 Guanosine 5'-O-(3-Thiotriphosphate) 5'-guanylylmethylenebisphosphonate Guanosine Triphosphate
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Diamantopoulos G S
Department of Cell Biology, Sciences III, University of Geneva, CH-1211 Geneva 4, Switzerland. [email protected]
Perez F
Goodson H V
Batelier G
Melki R
Kreis T E
Rickard J E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.144.1.99
Email
[email protected]
Published
1999-01-11
Pages
99-112
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148115
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.144.1.99 PMC Full Text View journal details
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