Inhibition of NF-kappa B activation in vitro and in vivo: role of 26S proteasome.

Methods in enzymology ·Vol. 300 ·1999-00-00 ·Pages 345-63

Grisham MB, Palombella VJ, Elliott PJ, Conner EM, Brand S, Wong HL, Pien C, Mazzola LM, Destree A, Parent L, Adams J

Abstract

It is becoming increasingly apparent that NF-kappa B plays a critical role in regulating the inflammatory response. Data obtained from studies in our laboratories demonstrate that the proteasome plays an important role in the inflammatory cascade by regulating the activation of NF-kappa B. Indeed, the availability of selective and orally active proteasome inhibitors should prove useful in delineating the roles of the proteasome and NF-kappa B in other pathophysiological conditions such as cancer and heart disease.

MeSH Terms

Acetylcysteine/analogs & derivatives,pharmacology Animals Arthritis/drug therapy Boronic Acids/pharmacology Cell Adhesion Molecules/biosynthesis Cytokines/biosynthesis Dipeptides/pharmacology Endothelium, Vascular/drug effects,metabolism Female HeLa Cells Humans Hypersensitivity, Delayed/drug therapy Jurkat Cells Leupeptins/pharmacology NF-kappa B/metabolism Peptide Hydrolases/drug effects Protease Inhibitors/pharmacology Proteasome Endopeptidase Complex Rats Rats, Inbred Lew T-Lymphocytes/drug effects

Chemicals

Boronic Acids Cell Adhesion Molecules Cytokines Dipeptides Leupeptins NF-kappa B PS 273 Protease Inhibitors lactacystin Peptide Hydrolases Proteasome Endopeptidase Complex ATP dependent 26S protease benzyloxycarbonylleucyl-leucyl-leucine aldehyde Acetylcysteine

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Grisham M B

Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.

Palombella V J

Elliott P J

Conner E M

Brand S

Wong H L

Pien C

Mazzola L M

Destree A

Parent L

Adams J

Article Info

Journal

Methods in enzymology

Abbr.

Methods Enzymol

ISSN

0076-6879

DOI

10.1016/s0076-6879(99)00140-8

Published

1999-00-00

Pages

345-63

Language

English

Region

United States

NLM ID

0212271

Subset

External Links

PubMed source DOI: 10.1016/s0076-6879(99)00140-8 View journal details

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Inhibition of NF-kappa B activation in vitro and in vivo: role of 26S proteasome.

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