Home LiteratureArticle Details

PMID: 9923682 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.

Nature ·Vol. 397 ·No. 6715 ·1999-01-14 ·Pages 176-80

Alm RA, Ling LS, Moir DT, King BL, Brown ED, Doig PC, Smith DR, Noonan B, Guild BC, deJonge BL, Carmel G, Tummino PJ, Caruso A, Uria-Nickelsen M, Mills DM, Ives C, Gibson R, Merberg D, Mills SD, Jiang Q, Taylor DE, Vovis GF, Trust TJ

Abstract

Helicobacter pylori, one of the most common bacterial pathogens of humans, colonizes the gastric mucosa, where it appears to persist throughout the host's life unless the patient is treated. Colonization induces chronic gastric inflammation which can progress to a variety of diseases, ranging in severity from superficial gastritis and peptic ulcer to gastric cancer and mucosal-associated lymphoma. Strain-specific genetic diversity has been proposed to be involved in the organism's ability to cause different diseases or even be beneficial to the infected host and to participate in the lifelong chronicity of infection. Here we compare the complete genomic sequences of two unrelated H. pylori isolates. This is, to our knowledge, the first such genomic comparison. H. pylori was believed to exhibit a large degree of genomic and allelic diversity, but we find that the overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genomes) of the two strains are quite similar. Between 6 to 7% of the genes are specific to each strain, with almost half of these genes being clustered in a single hypervariable region.

MeSH Terms

Duodenal Ulcer/microbiology Gene Expression Regulation, Bacterial Genome, Bacterial Helicobacter Infections/microbiology Helicobacter pylori/classification,genetics Humans Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Species Specificity

Authors & Affiliations

23 authors, click to expand affiliations / ORCID

Alm R A

Astra Research Center Boston, Cambridge, Massachusetts 02139-4239, USA. [email protected]

Ling L S

Moir D T

King B L

Brown E D

Doig P C

Smith D R

Noonan B

Guild B C

deJonge B L

Carmel G

Tummino P J

Caruso A

Uria-Nickelsen M

Mills D M

Ives C

Gibson R

Merberg D

Mills S D

Jiang Q

Taylor D E

Vovis G F

Trust T J

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/16495

[email protected]

Published

1999-01-14

Pages

176-80

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

AE001440, AE001441, AE001442, AE001443, AE001444, AE001445, AE001446, AE001447, AE001448, AE001449, AE001450, AE001451, AE001452, AE001453, AE001454, AE001455, AE001456, AE001457, AE001458, AE001459, AE001460, AE001461, AE001462, AE001463, AE001464, AE001465, AE001466, AE001467, AE001468, AE001469

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1038/16495 View journal details

Analysis Services

Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.

Contact

WeChat Official Account