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PMID: 9927658 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The specificity of interaction of archaeal transducers with their cognate sensory rhodopsins is determined by their transmembrane helices.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 3 ·1999-02-02 ·Pages 857-62

Zhang XN, Zhu J, Spudich JL

Abstract

Chimeras of the Halobacterium salinarum transducers HtrI and HtrII were constructed to study the structural determinants for their specific interaction with the phototaxis receptors sensory rhodopsins I and II (SRI and SRII), respectively. Interaction of receptors and transducers was assessed by two criteria: phototaxis responses by the cells and transducer-modulation of receptor photochemical reaction kinetics in membranes. Coexpression of HtrI with SRII or HtrII with SRI did not result in interaction by either criterion. Each receptor was coexpressed with chimeric transducers in which various domains of the two transducers were interchanged. The results show that the presence of the two transmembrane helices of HtrI in a chimera is necessary and sufficient for functional transducer complexation with SRI, i.e., for wild-type SRI photoreactions and attractant and 2-photon repellent phototaxis responses. Additionally, a previously demonstrated chaperone-like facilitation of SRI folding or stability by HtrI was shown to depend only on the two transmembrane helices of HtrI in chimeric transducers. Similarly, the two transmembrane helices of HtrII specify interaction with the repellent receptor SRII according to motility analysis and laser-flash spectroscopy. The results support a model in which the membrane domains of the receptor/transducer complexes, consisting of the seven helices of the receptor interacting with the four-helix bundle of the transducer dimer, produce SRI- and SRII-specific signals to the flagellar motor by means of interchangeable cytoplasmic domains.

MeSH Terms
Archaeal Proteins Bacterial Proteins/chemistry,metabolism Bacteriorhodopsins/chemistry,metabolism Carotenoids Cell Membrane/physiology,ultrastructure Halobacterium salinarum/physiology Halorhodopsins Kinetics Light Membrane Proteins/chemistry,metabolism Movement Protein Structure, Secondary Recombinant Fusion Proteins/chemistry,metabolism Sensory Rhodopsins Signal Transduction Time Factors
Chemicals
Archaeal Proteins Bacterial Proteins Halorhodopsins HtrII protein, Halobacterium salinarium Membrane Proteins Recombinant Fusion Proteins SRI protein, Halobacterium Sensory Rhodopsins htrI protein, Halobacterium salinarium sensory rhodopsin II protein, archaeal Carotenoids Bacteriorhodopsins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhang X N
Department of Microbiology and Molecular Genetics, The University of Texas Medical School, Houston, TX 77030, USA.
Zhu J
Spudich J L
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.3.857
Published
1999-02-02
Pages
857-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC15315
Subset
IM
Grants
NIGMS NIH HHS · R01 GM027750 · United States
NIGMS NIH HHS · R01-GM27750 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.3.857 PMC Full Text View journal details
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