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PMID: 995645 Published · ppublish English Journal Article

Cleavage of adeno-associated virus DNA with Sali,Psti and Haeii restriction endonucleases.

Nucleic acids research ·Vol. 3 ·No. 10 ·1976-10-00 ·Pages 2605-16

Maza LM, Carter BJ

Abstract

Duplex AAV-2 DNA was digested with SalI, PstI or HaeII restriction endonucleases and the cleavage sites were mapped. SalI cleaves AAV DNA at 0.310 map units, PstI at 0.106, 0.422 and 0.914 and the five HaeII sites were mapped at 0.110. 0.156, 0.181, 0.536 and 0.600 map units. These cleavage products will be useful for the isolation of specific regions from the AAV DNA, located outside of the stably transcribed region of the genome, and will also help to map more complex restriction enzyme cleavages.

MeSH Terms
Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Viral/analysis Endonucleases/metabolism Molecular Weight Parvoviridae/analysis Satellite Viruses/analysis
Chemicals
DNA, Viral Endonucleases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maza L M
Carter B J
References (11)
11 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.10.2605
Published
1976-10-00
Pages
2605-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343116
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.10.2605 PMC Full Text View journal details
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