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PMID: 9971742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly.

The Journal of cell biology ·Vol. 144 ·No. 3 ·1999-02-08 ·Pages 473-81

Pazour GJ, Dickert BL, Witman GB

Abstract

Dyneins are microtubule-based molecular motors involved in many different types of cell movement. Most dynein heavy chains (DHCs) clearly group into cytoplasmic or axonemal isoforms. However, DHC1b has been enigmatic. To learn more about this isoform, we isolated Chlamydomonas cDNA clones encoding a portion of DHC1b, and used these clones to identify a Chlamydomonas cell line with a deletion mutation in DHC1b. The mutant grows normally and appears to have a normal Golgi apparatus, but has very short flagella. The deletion also results in a massive redistribution of raft subunits from a peri-basal body pool (Cole, D.G., D.R. Diener, A.L. Himelblau, P.L. Beech, J.C. Fuster, and J.L. Rosenbaum. 1998. J. Cell Biol. 141:993-1008) to the flagella. Rafts are particles that normally move up and down the flagella in a process known as intraflagellar transport (IFT) (Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. USA. 90:5519-5523), which is essential for assembly and maintenance of flagella. The redistribution of raft subunits apparently occurs due to a defect in the retrograde component of IFT, suggesting that DHC1b is the motor for retrograde IFT. Consistent with this, Western blots indicate that DHC1b is present in the flagellum, predominantly in the detergent- and ATP-soluble fractions. These results indicate that DHC1b is a cytoplasmic dynein essential for flagellar assembly, probably because it is the motor for retrograde IFT.

MeSH Terms
Amino Acid Sequence Animals Biological Transport, Active Chlamydomonas reinhardtii/genetics,metabolism,ultrastructure Cloning, Molecular Cytoplasm/metabolism Cytoplasmic Dyneins Dyneins/chemistry,genetics,metabolism Flagella/metabolism,ultrastructure Gene Deletion Genes, Protozoan Golgi Apparatus/ultrastructure Microscopy, Electron Molecular Motor Proteins Molecular Sequence Data Protozoan Proteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid
Chemicals
DYNC2H1 protein, human Molecular Motor Proteins Protozoan Proteins Cytoplasmic Dyneins Dyneins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pazour G J
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
Dickert B L
Witman G B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.144.3.473
Published
1999-02-08
Pages
473-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132917
Subset
IM
Grants
NIGMS NIH HHS · GM30626 · United States
Databases
GENBANK
AF096277, AF106079
External Links
PubMed source DOI: 10.1083/jcb.144.3.473 PMC Full Text View journal details
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