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PMID: 9987690 Published · ppublish English Journal Article

Use of failed-fertilized oocytes for diagnostic zona binding purposes after sperm binding improvement with a modified medium.

Journal of assisted reproduction and genetics ·Vol. 16 ·No. 1 ·1999-01-00 ·Pages 24-9

Henkel R, Müller C, Stalf T, Schill WB, Franken DR

Abstract

Because the availability of prophase oocytes for zona binding testing is limited, we compared sperm binding to the zona of failed-fertilized intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) oocytes after incubation in a standard IVF medium and a specially composed binding improvement medium. Semen samples from nine patients and nine fertile donors were separated in parallel by the standard swim-up method in both media. Subsequently, hemizona assays were performed with prophase, failed-fertilized ICSI and IVF oocytes. Sperm separation resulted in a significantly higher sperm count (P < 0.01) and progressive motility (P = 0.018) in binding improvement medium. Moreover, spermatozoa coincubated with hemizonae (prophase, failed-fertilized ICSI and IVF oocytes) in binding improvement medium bound significantly more to hemizonae than in the controls (P < 0.01). However, the hemizona index did not differ. Thus, the limited number of human zonae can be increased by the use of oocytes that failed to fertilize during ICSI or IVF. This will lead to a qualitative improvement of the diagnostic spectrum in male-factor infertility.

MeSH Terms
Adolescent Adult Female Humans Infertility, Male/diagnosis Male Reproductive Techniques Sperm Count Sperm Motility Sperm-Ovum Interactions/physiology Spermatozoa/physiology Zona Pellucida/physiology
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Henkel R
Center of Dermatology and Andrology, Justus Liebig University Giessen, Germany.
Müller C
Stalf T
Schill W B
Franken D R
References (26)
26 references, click to expand
  1. Electron microscopic evidence on the acrosomal status of bound sperm and their penetration into human hemizonae pellucida after storage in a buffered salt solution.
    Andrologia. 1991 May-Jun;23(3):205-8 PMID: 1741483
  2. Defining the valid hemizona assay: accounting for binding variability within zonae pellucidae and within semen samples from fertile males.
    Fertil Steril. 1991 Dec;56(6):1156-61 PMID: 1743337
  3. Comparison of sperm binding potential of uninseminated, inseminated-unfertilized, and fertilized-noncleaved human oocytes under hemizona assay conditions.
    Mol Reprod Dev. 1991 Sep;30(1):56-61 PMID: 1781988
  4. Bicarbonate: carbon-dioxide regulation of sperm capacitation, hyperactivated motility, and acrosome reactions.
    Biol Reprod. 1991 May;44(5):806-13 PMID: 1907858
  5. Recurrent failure of in vitro fertilization: role of the hemizona assay in the sequential diagnosis of specific sperm-oocyte defects.
    Am J Obstet Gynecol. 1991 May;164(5 Pt 1):1210-5 PMID: 2035560
  6. Human preovulatory oocytes have a higher sperm-binding ability than immature oocytes under hemizona assay conditions: evidence supporting the concept of "zona maturation".
    Fertil Steril. 1991 Jun;55(6):1165-70 PMID: 2037109
  7. Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T-6 antibody.
    Fertil Steril. 1990 Sep;54(3):504-8 PMID: 2204554
  8. Time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in GnRH agonist/HMG-stimulated cycles.
    Hum Reprod. 1989 Nov;4(8):921-6 PMID: 2515208
  9. Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through direct activation of adenylate cyclase.
    J Biol Chem. 1985 Aug 15;260(17):9699-705 PMID: 2991260
  10. The activating effects of bicarbonate on sperm motility and respiration at ejaculation.
    Biochim Biophys Acta. 1987 Jun 22;924(3):519-29 PMID: 3036242
  11. Is the sperm-binding capability of the zona pellucida linked to its surface structure? A scanning electron microscopic study of human in vitro fertilization.
    J In Vitro Fert Embryo Transf. 1988 Jun;5(3):134-43 PMID: 3171321
  12. Molecular changes in the human zona pellucida associated with fertilization and human sperm-zona interactions.
    Ann N Y Acad Sci. 1988;541:621-32 PMID: 3195941
  13. The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential.
    Fertil Steril. 1988 Apr;49(4):688-97 PMID: 3350165
  14. Zona pellucida resistance to sperm penetration before the completion of human oocyte maturation.
    J Reprod Fertil. 1988 May;83(1):487-95 PMID: 3397956
  15. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid.
    Fertil Steril. 1985 Oct;44(4):493-8 PMID: 3902512
  16. Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization.
    Fertil Steril. 1983 May;39(5):594-602 PMID: 6404659
  17. Hemizona index (HZI) demonstrates excellent predictability when evaluating sperm fertilizing capacity in in vitro fertilization patients.
    J Androl. 1994 May-Jun;15(3):250-4 PMID: 7928667
  18. Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes.
    Hum Reprod. 1993 Dec;8(12):2180-4 PMID: 8150921
  19. Polarized expression of a band 3-related protein in mammalian sperm cells.
    Biol Reprod. 1993 Aug;49(2):326-31 PMID: 8373956
  20. Multiple activation of mouse sperm motility.
    Mol Reprod Dev. 1993 Sep;36(1):89-95 PMID: 8398134
  21. The ability of the hemizona assay to predict human fertilization in different and consecutive in-vitro fertilization cycles.
    Hum Reprod. 1993 Aug;8(8):1240-4 PMID: 8408521
  22. Are failed-fertilized human oocytes useful?
    Hum Reprod. 1993 Apr;8(4):503-7 PMID: 8501174
  23. Activation of mammalian sperm motility by regulation of microtubule sliding via cyclic adenosine 5'-monophosphate-dependent phosphorylation.
    Biol Reprod. 1995 Nov;53(5):1081-7 PMID: 8527511
  24. Sperm motility hyperactivation facilitates penetration of the hamster zona pellucida.
    Biol Reprod. 1995 Dec;53(6):1280-5 PMID: 8562682
  25. Influence of elevated pH levels on structural and functional characteristics of the human zona pellucida: functional morphological aspects.
    J Assist Reprod Genet. 1995 Oct;12(9):644-9 PMID: 8580665
  26. Zona pellucida as physiological trigger for the induction of acrosome reaction.
    Andrologia. 1998 Aug-Sep;30(4-5):275-80 PMID: 9739426
Article Info
Journal
Journal of assisted reproduction and genetics
Abbr.
J Assist Reprod Genet
ISSN
1058-0468
DOI
10.1023/a:1022541511875
Published
1999-01-00
Pages
24-9
Language
English
Region
Netherlands
NLM ID
9206495
PMCID
PMC3468209
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1022541511875 PMC Full Text View journal details
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