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Function of Escherichia coli MsbA, an essential ABC family transporter, in lipi…

Zhou(Z),White(K A),Polissi(A),Georgopoulos(C… J Biol Chem 1998-06-12

...hexa-acylated species of lipid A predominate in the outer membranes of wild type E. coli labeled for several generations...

Heterologous expression of human transketolase.

Schenk(G),Duggleby(R G),Nixon(P F) Int J Biochem Cell Biol 1998-07-10

...hexa-histidine tag. The highest specific activity was 13.5 U/mg and the K(m) values were 0.27 +/- 0.02 and 0.51 +/- 0.05...

Immunisation with recombinant AMA-1 protects mice against infection with Plasmo…

Anders(R F),Crewther(P E),Edwards(S),Margett… Vaccine 1998-06-29

...hexa-his tag, was purified by Ni-chelate chromatography and refolded in vitro in the presence of oxidised and reduced gl...

A rapid and sensitive bacterial assay to determine the inhibitory effect of 'in…

Ast(O),Jentsch(K D),Schramm(H J),Hunsmann(G)… J Virol Methods 1998-07-30

...hexa-peptides were screened for their ability to inhibit HIV-1 protease activity. One of these peptides showed a signifi...

Improved refolding of an immobilized fusion protein.

Stempfer(G),Höll-Neugebauer(B),Rudolph(R) Nat Biotechnol 1998-07-14

...hexa-arginie peptides were expressed in the cytosol of Escherichia coli in soluble form. The polycationic peptide moieti...

A fusion protein designed for noncovalent immobilization: stability, enzymatic …

Stempfer(G),Höll-Neugebauer(B),Kopetzki(E),R… Nat Biotechnol 1998-07-14

...hexa-arginine fusion peptide. This fusion protein can be directly adsorbed from crude cell extracts on polyanionic matri...

Enhanced metalloadsorption of bacterial cells displaying poly-His peptides.

Sousa(C),Cebolla(A),de Lorenzo(V) Nat Biotechnol 1998-07-07

The properties of Escherichia coli cells, acquired by cell surface presentation of one or two hexahistidine (His) cluste...

Conversion of N-sulfated glucosamine to N-sulfated mannosamine in an unsaturate…

Yamada(S),Watanabe(M),Sugahara(K) Carbohydr Res 1998-10-29

...HexA(2-O-sulfate)(alpha 1-4)GlcN(2-N-,6-O-disulfate) (delta HexA and GlcN represent 4-deoxy-alpha-L-threo-hex-4-enepyran...

Expression of recombinant psoriasis-associated fatty acid binding protein in Es…

Vorum(H),Madsen(P),Svendsen(I),Cells(J E),Ho… Electrophoresis 1998-11-09

...hexa-His tag followed by a factor Xa cleavage site. The recombinant protein was expressed at a level of about 30% of the...

Determinants of recombinant production of antimicrobial cationic peptides and c…

Zhang(L),Falla(T),Wu(M),Fidai(S),Burian(J),K… Biochem Biophys Res Commun 1998-07-31

...hexa histidine domain. The improved system showed high level expression and simplified downstream purification. The acti...

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