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PMID: 10037799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.

The Journal of cell biology ·Vol. 144 ·No. 4 ·1999-02-22 ·Pages 789-801

Banerji S, Ni J, Wang SX, Clasper S, Su J, Tammi R, Jones M, Jackson DG

Abstract

The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and embryonic morphogenesis. Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Currently, however, the identities of HA binding molecules which control this pathway are unknown. Here we describe the first such molecule, LYVE-1, which we have identified as a major receptor for HA on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

MeSH Terms
Amino Acid Sequence Animals Base Sequence COS Cells Cell Membrane/metabolism Cells, Cultured Cloning, Molecular DNA Primers/genetics DNA, Complementary/genetics Glycoproteins/chemistry,genetics,metabolism Humans Hyaluronan Receptors/chemistry,genetics,metabolism Hyaluronic Acid/metabolism Lymphatic System/metabolism Molecular Sequence Data RNA, Messenger/genetics,metabolism Sequence Homology, Amino Acid Tissue Distribution Transfection Vesicular Transport Proteins
Chemicals
DNA Primers DNA, Complementary Glycoproteins Hyaluronan Receptors LYVE1 protein, human RNA, Messenger Vesicular Transport Proteins Hyaluronic Acid
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Banerji S
University of Oxford, Molecular Immunology Group, Nuffield Department of Medicine, John Radcliff Hospital, Headington, Oxford OX3 9DU, United Kingdom.
Ni J
Wang S X
Clasper S
Su J
Tammi R
Jones M
Jackson D G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.144.4.789
Published
1999-02-22
Pages
789-801
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132933
Subset
IM
Databases
GENBANK
AF118108
External Links
PubMed source DOI: 10.1083/jcb.144.4.789 PMC Full Text View journal details
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