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PMID: 10074090 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The polar flagellar motor of Vibrio cholerae is driven by an Na+ motive force.

Journal of bacteriology ·Vol. 181 ·No. 6 ·1999-03-00 ·Pages 1927-30

Kojima S, Yamamoto K, Kawagishi I, Homma M

Abstract

Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence of V. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes of the Na+-driven polar flagellar motor of Vibrio alginolyticus were found in the genome database of V. cholerae. The energy source of its flagellar motor was investigated. We examined the Na+ dependence and the sensitivity to the Na+ motor-specific inhibitor of the motility of the V. cholerae strains and present the evidence that the polar flagellar motor of V. cholerae is driven by an Na+ motive force.

MeSH Terms
Flagella/metabolism,physiology Genes, Bacterial Ion Transport Molecular Motor Proteins/genetics,metabolism Movement Osmolar Concentration Sodium/metabolism Sodium-Potassium-Exchanging ATPase/metabolism Species Specificity Vibrio/genetics,metabolism,physiology Vibrio cholerae/genetics,metabolism,physiology
Chemicals
Molecular Motor Proteins Sodium Sodium-Potassium-Exchanging ATPase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kojima S
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
Yamamoto K
Kawagishi I
Homma M
References (40)
40 references, click to expand
  1. Identity of hemolysins produced by Vibrio cholerae non-O1 and V. cholerae O1, biotype El Tor.
    Infect Immun. 1986 Mar;51(3):927-31 PMID: 3081448
  2. Function of protonatable residues in the flagellar motor of Escherichia coli: a critical role for Asp 32 of MotB.
    J Bacteriol. 1998 May;180(10):2729-35 PMID: 9573160
  3. The sodium cycle. III. Vibrio alginolyticus resembles Vibrio cholerae and some other vibriones by flagellar motor and ribosomal 5S-RNA structures.
    Biochim Biophys Acta. 1986 Jul 23;850(3):466-72 PMID: 3730372
  4. Bacterial motility: membrane topology of the Escherichia coli MotB protein.
    Science. 1988 Jan 15;239(4837):276-8 PMID: 2447650
  5. Amiloride, a specific inhibitor for the Na+-driven flagellar motors of alkalophilic Bacillus.
    J Biol Chem. 1988 Jun 15;263(17):8215-9 PMID: 3372520
  6. Na+-driven bacterial flagellar motors.
    J Bioenerg Biomembr. 1989 Dec;21(6):705-16 PMID: 2687262
  7. The MotA protein of E. coli is a proton-conducting component of the flagellar motor.
    Cell. 1990 Feb 9;60(3):439-49 PMID: 2154333
  8. Specific inhibition of the Na(+)-driven flagellar motors of alkalophilic Bacillus strains by the amiloride analog phenamil.
    J Bacteriol. 1990 Mar;172(3):1634-9 PMID: 2155207
  9. Surface-induced swarmer cell differentiation of Vibrio parahaemolyticus.
    Mol Microbiol. 1990 Jul;4(7):1057-62 PMID: 2233248
  10. Mutant MotB proteins in Escherichia coli.
    J Bacteriol. 1991 Jul;173(13):4049-55 PMID: 2061285
  11. Roles of motility and flagellar structure in pathogenicity of Vibrio cholerae: analysis of motility mutants in three animal models.
    Infect Immun. 1991 Aug;59(8):2727-36 PMID: 1855990
  12. Evidence for interactions between MotA and MotB, torque-generating elements of the flagellar motor of Escherichia coli.
    J Bacteriol. 1991 Nov;173(21):7033-7 PMID: 1938906
  13. Mutations in the MotA protein of Escherichia coli reveal domains critical for proton conduction.
    J Mol Biol. 1991 Oct 20;221(4):1433-42 PMID: 1719217
  14. Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces.
    Nature. 1992 Jan 9;355(6356):182-4 PMID: 1309599
  15. Cholera isolates in relation to the "eighth pandemic".
    Lancet. 1993 Oct 9;342(8876):926 PMID: 8105183
  16. Na(+)-translocating NADH-quinone reductase of marine and halophilic bacteria.
    J Bioenerg Biomembr. 1993 Aug;25(4):385-91 PMID: 8226720
  17. MotY, a component of the sodium-type flagellar motor.
    J Bacteriol. 1994 Jul;176(14):4219-25 PMID: 8021208
  18. MotX, the channel component of the sodium-type flagellar motor.
    J Bacteriol. 1994 Oct;176(19):5988-98 PMID: 7928960
  19. Motility protein interactions in the bacterial flagellar motor.
    Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1970-4 PMID: 7892209
  20. Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus.
    FEBS Lett. 1995 Apr 17;363(1-2):75-7 PMID: 7729558
  21. Tryptophan-scanning mutagenesis of MotB, an integral membrane protein essential for flagellar rotation in Escherichia coli.
    Biochemistry. 1995 Jul 18;34(28):9166-71 PMID: 7619816
  22. Membrane topology of the MotA protein of Escherichia coli.
    J Mol Biol. 1995 Aug 11;251(2):237-42 PMID: 7643400
  23. Isolation of the polar and lateral flagellum-defective mutants in Vibrio alginolyticus and identification of their flagellar driving energy sources.
    J Bacteriol. 1995 Sep;177(17):5158-60 PMID: 7665498
  24. Amino acids of the cholera toxin from Vibrio cholerae O37 strain S7 which differ from those of strain O1.
    Gene. 1995 Sep 22;163(1):155-6 PMID: 7557467
  25. How bacteria sense and swim.
    Annu Rev Microbiol. 1995;49:489-522 PMID: 8561469
  26. Cloning and characterization of motY, a gene coding for a component of the sodium-driven flagellar motor in Vibrio alginolyticus.
    J Bacteriol. 1996 Apr;178(8):2409-15 PMID: 8636046
  27. Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression.
    Infect Immun. 1996 Jun;64(6):2246-55 PMID: 8675334
  28. Chemical and physical properties of cholera exo-enterotoxin (choleragen) and its spontaneously formed toxoid (choleragenoid).
    Biochim Biophys Acta. 1972 Jan 26;257(1):158-66 PMID: 5009826
  29. Induction of swarming in Vibrio parahaemolyticus.
    Arch Microbiol. 1974;101(4):357-63 PMID: 4455130
  30. The mechanism of swarming of Vibrio alginolyticus.
    Arch Microbiol. 1975 Jun 20;104(1):67-71 PMID: 239659
  31. Role of chemotaxis in the association of motile bacteria with intestinal mucosa: chemotactic responses of Vibrio cholerae and description of motile nonchemotactic mutants.
    Infect Immun. 1981 Oct;34(1):215-21 PMID: 7298183
  32. Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment.
    Infect Immun. 1981 Oct;34(1):90-7 PMID: 7298194
  33. Scanning electron microscope observation of the swarming phenomenon of Vibrio parahaemolyticus.
    J Bacteriol. 1982 May;150(2):956-9 PMID: 7068539
  34. Structure and cell envelope associations of flagellar basal complexes of Vibrio cholerae and Campylobacter fetus.
    Can J Microbiol. 1984 Mar;30(3):322-33 PMID: 6426766
  35. Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli.
    J Bacteriol. 1984 Sep;159(3):991-9 PMID: 6090403
  36. Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium.
    J Bacteriol. 1997 Aug;179(16):5104-10 PMID: 9260952
  37. Residues of the cytoplasmic domain of MotA essential for torque generation in the bacterial flagellar motor.
    J Mol Biol. 1997 Oct 24;273(2):428-39 PMID: 9344750
  38. TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae.
    Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):730-4 PMID: 9435261
  39. Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus.
    FEBS Lett. 1998 Jan 30;422(2):240-2 PMID: 9490015
  40. Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane.
    J Bacteriol. 1986 Apr;166(1):244-52 PMID: 3007435
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.6.1927-1930.1999
Published
1999-03-00
Pages
1927-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93596
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.181.6.1927-1930.1999 PMC Full Text View journal details
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