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PMID: 8675334 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression.

Infection and immunity ·Vol. 64 ·No. 6 ·1996-06-00 ·Pages 2246-55

Gardel CL, Mekalanos JJ

Abstract

Motility is thought to contribute to the virulence of Vibrio cholerae, but the role it plays in pathogenesis is not completely understood. To investigate the influence of motility on virulence gene expression and intestinal colonization, we have isolated mutants with altered swarming abilities in soft agar medium. Both spontaneous hyperswarmer (exhibiting faster swarm rates) and spontaneous or transposon-induced nonmotile mutants of strain 0395 were obtained. Surprisingly, we found that two of three classes of hyperswarmer mutants were defective in autoagglutination, a phenotype associated with expression of toxin-coregulated pili (TCP), an essential ToxR-regulated colonization factor of V. cholerae. In contrast, nonmotile mutants exhibited autoagglutination under growth conditions that normally repress this phenotype. Further characterization of mutant strains revealed differences in the expression of other virulence determinants. Class I hyperswarmer mutants were defective in production of TCP, cholera toxin, and a cell-associated hemolysin but showed increased levels of protease and fucose-sensitive hemagglutinin. All nonmotile mutants examined, including those with insertions in a sequence homologous to motB, exhibited increased expression of TCP pilin, cholera toxin, and cell-associated hemolysin but dramatically decreased levels of fucose-sensitive hemagglutinin and HEp-2 adhesins. In general, nonmotile mutants displayed few or no defects in intestinal colonization, while class I hypermotile mutants were highly defective in colonization. These results suggest that the motility phenotype of V. cholerae is tightly coupled to the expression of multiple ToxR-regulated and non-ToxR-regulated virulence determinants.

MeSH Terms
Agglutination Amino Acid Sequence Bacterial Adhesion Bacterial Outer Membrane Proteins/analysis Base Sequence Cholera Toxin/analysis Endopeptidases/biosynthesis Fimbriae Proteins Hemolysin Proteins/biosynthesis Humans Molecular Sequence Data Movement Mutation Phenotype Vibrio cholerae/pathogenicity Virulence
Chemicals
Bacterial Outer Membrane Proteins Hemolysin Proteins Fimbriae Proteins Cholera Toxin Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gardel C L
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mekalanos J J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.6.2246-2255.1996
Published
1996-06-00
Pages
2246-55
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC174063
Subset
IM
Grants
NIAID NIH HHS · AI 18045-13 · United States
External Links
PubMed source DOI: 10.1128/iai.64.6.2246-2255.1996 PMC Full Text View journal details
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