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PMID: 8005659 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Vibrio cholerae toxin-coregulated-pilus gene tcpI encodes a homolog of methyl-accepting chemotaxis proteins.

Infection and immunity ·Vol. 62 ·No. 7 ·1994-07-00 ·Pages 2669-78

Harkey CW, Everiss KD, Peterson KM

Abstract

Virulence gene activation in Vibrio cholerae is under the control of the ToxR-ToxT regulatory cascade. The ToxR regulon consists of genes required for toxin-coregulated-pilus (TCP) biogenesis, accessory colonization factor genes, cholera toxin genes, and ToxR-activated genes (tag) of unknown function. The tagB gene was isolated by using a tagB::TnphoA fusion junction to probe a V. cholerae )395 bacteriophage lambda library. Nucleotide sequence analysis revealed that tagB is identical to tcpI, a gene which encodes a protein that negatively regulates the synthesis of the major pilin subunit of TCP (TcpA). Our results show that the tcpI gene encodes a 620-amino-acid protein that shares extensive sequence similarity with the highly conserved signaling domain in methyl-accepting chemotaxis proteins. Expression of tcpI in Escherichia coli results in the synthesis of a 71-kDa polypeptide that becomes localized to the inner membrane. Similarly, TcpI-PhoA alkaline phosphatase activity is enriched in V. cholerae inner membrane preparations. Colonies of V. cholerae tcpI::TnphoA mutant cells display increased swarming on solid media when compared with those of the parental V. cholerae O395. Taken together, these observations suggest that TcpI may play a dual role in promoting vibrio colonization of the small bowel. In response to the appropriate environmental signal(s), TcpI permits maximum expression of tcpA while simultaneously reducing vibrio chemotaxis-directed motility. We believe coordinate regulation of colonization and motility determinants, in such a fashion, facilitates efficient V. cholerae microcolony formation.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/biosynthesis Bacterial Proteins/biosynthesis Base Sequence Cell Compartmentation Cell Movement/genetics Cloning, Molecular Escherichia coli/genetics Fimbriae Proteins Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Genes, Regulator/genetics Membrane Proteins/biosynthesis,genetics Methyl-Accepting Chemotaxis Proteins Molecular Sequence Data Mutagenesis, Insertional Protein Conformation Sequence Analysis, DNA Sequence Homology, Amino Acid Transcriptional Activation Vibrio cholerae/genetics
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Membrane Proteins Methyl-Accepting Chemotaxis Proteins TcpA protein, Vibrio cholerae Fimbriae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harkey C W
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.
Everiss K D
Peterson K M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.7.2669-2678.1994
Published
1994-07-00
Pages
2669-78
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC302867
Subset
IM
Grants
NIAID NIH HHS · AI28502 · United States
Databases
GENBANK
L25659
External Links
PubMed source DOI: 10.1128/iai.62.7.2669-2678.1994 PMC Full Text View journal details
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