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PMID: 10085293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p.

The Journal of cell biology ·Vol. 144 ·No. 5 ·1999-03-08 ·Pages 947-61

Lee L, Klee SK, Evangelista M, Boone C, Pellman D

Abstract

Alignment of the mitotic spindle with the axis of cell division is an essential process in Saccharomyces cerevisiae that is mediated by interactions between cytoplasmic microtubules and the cell cortex. We found that a cortical protein, the yeast formin Bni1p, was required for spindle orientation. Two striking abnormalities were observed in bni1Delta cells. First, the initial movement of the spindle pole body (SPB) toward the emerging bud was defective. This phenotype is similar to that previously observed in cells lacking the kinesin Kip3p and, in fact, BNI1 and KIP3 were found to be in the same genetic pathway. Second, abnormal pulling interactions between microtubules and the cortex appeared to cause preanaphase spindles in bni1Delta cells to transit back and forth between the mother and the bud. We therefore propose that Bni1p may localize or alter the function of cortical microtubule-binding sites in the bud. Additionally, we present evidence that other bipolar bud site determinants together with cortical actin are also required for spindle orientation.

MeSH Terms
Actins/metabolism Base Sequence Binding Sites DNA Primers Fungal Proteins/metabolism,physiology Microfilament Proteins/metabolism,physiology Microscopy, Fluorescence Microtubules Molecular Sequence Data Saccharomyces cerevisiae/cytology,genetics,physiology Saccharomyces cerevisiae Proteins Spindle Apparatus/physiology
Chemicals
Actins BUD6 protein, S cerevisiae Bni1 protein, S cerevisiae DNA Primers Fungal Proteins Microfilament Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee L
Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Klee S K
Evangelista M
Boone C
Pellman D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.144.5.947
Published
1999-03-08
Pages
947-61
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148193
Subset
IM
Grants
NIGMS NIH HHS · GM55772 · United States
External Links
PubMed source DOI: 10.1083/jcb.144.5.947 PMC Full Text View journal details
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