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PMID: 10094696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RsaL, a novel repressor of virulence gene expression in Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 181 ·No. 7 ·1999-04-00 ·Pages 2175-84

de Kievit T, Seed PC, Nezezon J, Passador L, Iglewski BH

Abstract

As components of a Pseudomonas aeruginosa quorum-sensing system, LasR and PAI-1 globally regulate expression of multiple virulence determinants, as well as the second P. aeruginosa quorum-sensing system. To date, no information exists on negative regulation of the quorum-sensing cascade in P. aeruginosa. Here we describe a novel gene, rsaL, which is located downstream from lasR and transcribed antisense relative to lasR. In P. aeruginosa, overexpression of rsaL results in reduced lasB expression and decreased elastase activity. With the use of a six-His protein fusion system, we demonstrate that rsaL encodes an 11-kDa protein. Direct quantitation of PAI-1 levels in cultures and studies utilizing Escherichia coli lambda lysogens carrying lacZ transcriptional fusions reveal that RsaL specifically represses transcription of the PAI-1 autoinducer synthase gene, lasI. RsaL's repressive effect on lasI and the associated decrease in elastase activity have important implications for the expression of all LasR-PAI-1-dependent virulence genes and the overall pathogenicity of P. aeruginosa.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence DNA, Bacterial DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Pseudomonas aeruginosa/genetics,pathogenicity Repressor Proteins/genetics Trans-Activators/genetics,metabolism Virulence
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins LasI protein, Pseudomonas aeruginosa LasR protein, Pseudomonas aeruginosa Repressor Proteins RhlR protein, Pseudomonas aeruginosa Trans-Activators rsaL protein, Pseudomonas aeruginosa
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
de Kievit T
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642, USA.
Seed P C
Nezezon J
Passador L
Iglewski B H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.7.2175-2184.1999
Published
1999-04-00
Pages
2175-84
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93631
Subset
IM
Grants
NIAID NIH HHS · T32 AI007362 · United States
NIAID NIH HHS · 5T32AI07362 · United States
PHS HHS · R01A 133713-04 · United States
Databases
GENBANK
M59425
External Links
PubMed source DOI: 10.1128/JB.181.7.2175-2184.1999 PMC Full Text View journal details
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