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PMID: 1012267 Published · ppublish English Journal Article

Recombination of bacteriophage T7 in vivo.

Molecular & general genetics : MGG ·Vol. 149 ·No. 1 ·1976-11-24 ·Pages 63-71

Powling A, Knippers R

Abstract

Most recombination following infection with T7 was found to coincide with the time of most repid DNA synthesis, at about 20 min after infection at 30 degrees in minimal medium. Recombining DNA was investigated electron microscopically. Multiply branched DNA structures were observed after infection with T7 wild type, gene 3-, gene 6- and genes 3-, 6- phage, but not after infection with T7 gene 5- phage. Evidence is presented indicating that these structures are T7 DNA molecules in the process of recombining. The detailed structures of these recombinational intermediates suggest mechanisms by which T7 DNA initiates recombination.

MeSH Terms
Coliphages DNA Replication DNA, Viral/biosynthesis Genes Microscopy, Electron Molecular Conformation Mutation Recombination, Genetic Time Factors
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Powling A
Knippers R
References (25)
25 references, click to expand
  1. Initiation and reinitiation of DNA synthesis during replication of bacteriophage T7.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):998-1002 PMID: 4554539
  2. Amber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis.
    J Virol. 1967 Aug;1(4):779-92 PMID: 4912235
  3. Regions of single-stranded DNA in the growing points of replicating bacteriophage T7 chromosomes.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2682-6 PMID: 4560695
  4. Origin of concatemeric T7 DNA.
    Nat New Biol. 1972 Oct 18;239(94):197-201 PMID: 4507727
  5. A genetic study of the temperate coliphage.
    Virology. 1955 Nov;1(4):424-43 PMID: 13274744
  6. Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction.
    J Biol Chem. 1972 Jan 10;247(1):311-8 PMID: 4553438
  7. Genetic recombination of bacteriophage T7 DNA in vitro.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):692-6 PMID: 1062779
  8. The vegetative state in the life cycle of bacteriophage: evidence for its occurrence and its genetic characterization.
    Cold Spring Harb Symp Quant Biol. 1953;18:3-11 PMID: 13168958
  9. An electron microscopic analysis of pathways for bacteriophage T4 DNA recombination.
    J Mol Biol. 1973 Nov 25;81(1):1-16 PMID: 4588574
  10. Structure of the replicating DNA from bacteriophage T4.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3049-53 PMID: 4943552
  11. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.
    Biochim Biophys Acta. 1973 Apr 11;299(4):516-20 PMID: 4575181
  12. Genotypes produced by individual recombination events involving bacteriophage f1.
    J Mol Biol. 1971 May 28;58(1):133-51 PMID: 5088923
  13. Some functions involved in bacteriophage T7 genetic recombination.
    Mol Gen Genet. 1974;134(2):173-80 PMID: 4617162
  14. Polynucleotide ligase in bacteriophage T4D recombination.
    Genetics. 1972 Oct;72(2):187-203 PMID: 4567285
  15. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  16. Molecular and genetic recombination of bacteriophage T4.
    Annu Rev Genet. 1975;9:213-44 PMID: 1108766
  17. Recombinant DNA molecules of bacteriophage phi chi174.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):235-9 PMID: 1054500
  18. Bacteriophage T7.
    Science. 1972 Apr 28;176(4033):367-76 PMID: 4554613
  19. The involvement of genes 3,4,5 and 6 in genetic recombination in bacteriophage T7.
    Virology. 1975 May;65(1):281-5 PMID: 1146231
  20. Formation of concatemeric DNA in bacteriophage T7-infected bacteria.
    Virology. 1975 Jun;65(2):455-68 PMID: 1093318
  21. Physical mapping of the early region of bacteriophage T7 DNA.
    J Mol Biol. 1973 Sep 15;79(2):249-65 PMID: 4586410
  22. An intermediate in the replication of bacteriophage T7 DNA molecules.
    J Mol Biol. 1969 Sep 28;44(3):459-75 PMID: 5346051
  23. The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.
    J Mol Biol. 1969 Oct 28;45(2):237-63 PMID: 5367027
  24. The genetics and physiology of bacteriophage T7.
    Virology. 1969 Nov;39(3):562-74 PMID: 4902069
  25. Branched DNA molecules: intermediates in T4 recombination.
    J Mol Biol. 1971 Aug 28;60(1):131-49 PMID: 4937189
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00275961
Published
1976-11-24
Pages
63-71
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00275961
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