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PMID: 101529 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Classes of Anabaena variabilis mutants with oxygen-sensitive nitrogenase activity.

Journal of bacteriology ·Vol. 136 ·No. 2 ·1978-11-00 ·Pages 688-92

Haury JF, Wolk CP

Abstract

Mutants of Anabaena variabilis deficient in the envelope glycolipids of heterocysts have no or very low nitrogenase activity when assayed aerobically. Revertants capable of aerobic growth on N2 have increased quantities of these glycolipids. Among mutants which require fixed nitrogen for growth in air and which have a normal complement of glycolipids, one expresses high nitrogenase activity at low oxygen tension. Three others show high nitrogenase activity only in the presence of dithionite and are therefore impaired in electron transfer.

MeSH Terms

Cyanobacteria/analysis,enzymology Dithionite/pharmacology Glycolipids/physiology Membrane Lipids/physiology Mutation Nitrogenase/antagonists & inhibitors,metabolism Oxygen/pharmacology

Chemicals

Glycolipids Membrane Lipids Dithionite Nitrogenase Oxygen

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Haury J F

Wolk C P

References (14)

14 references, click to expand

Properties of heterocysts isolated with colloidal silica.
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Formation of glutamine from [13n]ammonia, [13n]dinitrogen, and [14C]glutamate by heterocysts isolated from Anabaena cylindrica.
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Modes of reduction of nitrogen in heterocysts isolated from Anabaena species.
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Effects of L-methionine-DL-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica.
Biochem Biophys Res Commun. 1975 Aug 4;65(3):846-56 PMID: 239712
Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.
J Bacteriol. 1977 Mar;129(3):1556-62 PMID: 403180
Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.
J Bacteriol. 1975 Mar;121(3):759-65 PMID: 1090602
Nitrogen fixation by photosynthetic microorganisms.
Annu Rev Microbiol. 1973;27:283-316 PMID: 4201690
Autoradiographic localization of 13N after fixation of 13N-labeled nitrogen gas by a heterocyst-forming blue-green alga.
J Cell Biol. 1974 May;61(2):440-53 PMID: 4208073
Structural studies on the glycolipids from the envelope of the heterocyst of Anabaena cylindrica.
Biochemistry. 1973 Feb 27;12(5):791-8 PMID: 4631370
Is the heterocyst the site of nitrogen fixation in blue-green algae?
Nature. 1968 Nov 23;220(5169):810-2 PMID: 4880705
Nitrogenase activity in heterocysts of blue-green algae.
Nature. 1969 Oct 18;224(5216):226-8 PMID: 4981122
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.
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Localization of an uptake hydrogenase in anabaena.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.136.2.688-692.1978

Published

1978-11-00

Pages

688-92

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC218594

Subset

External Links

PubMed source DOI: 10.1128/jb.136.2.688-692.1978 PMC Full Text View journal details

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Classes of Anabaena variabilis mutants with oxygen-sensitive nitrogenase activity.

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