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PMID: 1090602 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.

Journal of bacteriology ·Vol. 121 ·No. 3 ·1975-03-00 ·Pages 759-65

St John RT, Johnston HM, Seidman C, Garfinkel D, Gordon JK, Shah VK, Brill WJ

Abstract

Selected mutant strains of Klebsiella pneumoniae that are unable to fix nitrogen have been characterized according to nitrogenase component activity as well as antigenic cross-reacting material. The lesions in these strains have been mapped by transduction, and the results indicate that there are at least five genes specifically responsible for nitrogen fixation in vivo. Besides genes that specify the structure of the two nitrogenase components, there is a gene for a factor that is required for component I activity and a gene that codes for a factor possibly involved in electron transport to component II. A mutation in another site does not allow the organism to produce either of the nitrogenase components. All of these genes are co-transducible with the gene that specifics the structure of histidinol dehydrogenase.

MeSH Terms
Alcohol Oxidoreductases/metabolism Amino Alcohols/metabolism Chromosome Mapping Crosses, Genetic Electron Transport Enzyme Repression Genes Histidine/biosynthesis,metabolism Klebsiella pneumoniae/enzymology,growth & development,metabolism Mutagens Mutation Nitrogen/metabolism Nitrogen Fixation Nitrogenase/metabolism Nitrosoguanidines Phenotype Quaternary Ammonium Compounds/metabolism Recombination, Genetic Transduction, Genetic
Chemicals
Amino Alcohols Mutagens Nitrosoguanidines Quaternary Ammonium Compounds Histidine Alcohol Oxidoreductases Nitrogenase Nitrogen
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
St John R T
Johnston H M
Seidman C
Garfinkel D
Gordon J K
Shah V K
Brill W J
References (30)
30 references, click to expand
  1. Detection of nitrogenase components and other nonheme iron proteins in polyacrylamide gels.
    Anal Biochem. 1974 Jul;60(1):237-41 PMID: 4136629
  2. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  3. Mutant of Azotobacter vinelandii that hyperproduces nitrogenase component II.
    J Bacteriol. 1974 Feb;117(2):917-9 PMID: 4204445
  4. Electron paramagnetic resonance of nitrogenase and nitrogenase components from Clostridium pasteurianum W5 and Azotobacter vinelandii OP.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3142-5 PMID: 4343957
  5. Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3242-6 PMID: 4399835
  6. The nitrogen fixation genes.
    Nature. 1972 Oct 27;239(5374):495-9 PMID: 4563018
  7. Control of nitrogenase synthesis in Klebsiella pneumoniae.
    J Gen Microbiol. 1973 Nov;79(1):103-17 PMID: 4589994
  8. Effect of molybdenum starvation and tungsten on the synthesis of nitrogenase components in Klebsiella pneumonia.
    J Bacteriol. 1974 Jun;118(3):986-9 PMID: 4598014
  9. On the origin and direction of replication of the Escherichia coli K12 chromosome.
    J Mol Biol. 1968 Mar 28;32(3):611-29 PMID: 4868760
  10. Transfer of nitrogen-fixation genes by conjugation in Klebsiella pneumoniae.
    Nature. 1971 Nov 5;234(5323):47-8 PMID: 4942884
  11. Mutants of Azotobacter vinelandii unable to fix nitrogen.
    Biochim Biophys Acta. 1969 Jun 17;184(1):99-105 PMID: 5791118
  12. Effect of amino acids on the nitrogenase system of Klebsiella pneumoniae.
    J Bacteriol. 1966 Sep;92(3):618-22 PMID: 5922536
  13. Mutants that produce nitrogenase in the presence of ammonia.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3501-3 PMID: 16592035
  14. Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes.
    J Biol Chem. 1973 Jun 25;248(12):4334-44 PMID: 4145798
  15. Activation of transcription of hut DNA by glutamine synthetase.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):225-9 PMID: 4149344
  16. Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis.
    J Bacteriol. 1974 Nov;120(2):815-21 PMID: 4156359
  17. Activation of inactive nitrogenase by acid-treated component I.
    J Bacteriol. 1974 Nov;120(2):697-701 PMID: 4218230
  18. UGA nonsense mutations in Salmonella typhimurium.
    J Bacteriol. 1970 May;102(2):467-75 PMID: 4315894
  19. Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra.
    Biochim Biophys Acta. 1973 Jan 18;292(1):246-55 PMID: 4350172
  20. Nitrogenase V. The effect of Mo, W and V on the synthesis of nitrogenase components in Azotobacter vinelandii.
    Biochim Biophys Acta. 1974 Aug 7;362(1):160-6 PMID: 4422774
  21. Genetic transfer of nitrogen fixation from Klebsiella pneumoniae to Escherichia coli.
    Nature. 1972 May 12;237(5350):102-3 PMID: 4555442
  22. Bacteriophage P1 derivatives with bacterial genes: a heterozygote enrichment method for the selection of p1dpro lysogens.
    Virology. 1973 Jun;53(2):471-5 PMID: 4576347
  23. Chromosomal integration of Klebsiella nitrogen fixation genes in Escherichia coli.
    J Gen Microbiol. 1974 Jan;80(1):227-39 PMID: 4595005
  24. Regulation of nitrogenase synthesis by oxygen in Klebsiella pneumoniae.
    J Bacteriol. 1974 Jul;119(1):266-9 PMID: 4600701
  25. Derepression of nitrogenase synthesis in the presence of excess NH4+.
    Biochem Biophys Res Commun. 1974 Aug 5;59(3):967-71 PMID: 4606417
  26. Enriched selection of dominant mutations: histidine operator mutations.
    J Bacteriol. 1971 Aug;107(2):578-9 PMID: 4939768
  27. Nitrogenaseless mutants of Azotobacter vinelandii.
    Proc Natl Acad Sci U S A. 1970 Jan;65(1):74-80 PMID: 5263763
  28. Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1174-7 PMID: 5288365
  29. Interspecific transformation in Azotobacter.
    J Gen Microbiol. 1965 Oct;41(1):1-6 PMID: 5883695
  30. Glutamine synthetase and ammonium regulation of nitrogenase synthesis in Klebsiella.
    Nature. 1974 Oct 11;251(5475):481-5 PMID: 4153588
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.121.3.759-765.1975
Published
1975-03-00
Pages
759-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246000
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.121.3.759-765.1975 PMC Full Text View journal details
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