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PMID: 4315894 Published · ppublish English Journal Article

UGA nonsense mutations in Salmonella typhimurium.

Journal of bacteriology ·Vol. 102 ·No. 2 ·1970-05-00 ·Pages 467-75

Roth JR

Abstract

Salmonella typhimurium strain LT-2 carries a weak UGA suppressor activity. This activity prevents the detection of some UGA mutants as auxotrophs and probably accounts for the rarity of his UGA mutants in this strain. A selection method is described which permits the isolation of these rare his UGA mutants. Map distribution of his UGA mutations is normal, and their polarity effects are indistinguishable from the polarity effects of amber and ochre mutations at similar locations. Isolation and properties of a prototrophic his UGA mutant are described. UGA mutants are common among lac mutants isolated from Salmonella strains carrying an F'lac episome. Apparently the suppressor activity is insufficient to prevent detection of lac UGA mutants. It is not yet clear whether the suppressor activity plays an important role in normal cell physiology.

MeSH Terms
Chromosome Mapping Culture Media Galactosidases/metabolism Genetic Code Genetics, Microbial Histidine/metabolism Mutation Penicillins Phosphoric Monoester Hydrolases/metabolism Recombination, Genetic Salmonella Phages Salmonella typhimurium/classification,enzymology,isolation & purification,metabolism Selection, Genetic Suppression, Genetic Transduction, Genetic
Chemicals
Culture Media Penicillins Histidine Phosphoric Monoester Hydrolases Galactosidases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Roth J R
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.102.2.467-475.1970
Published
1970-05-00
Pages
467-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247572
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.102.2.467-475.1970 PMC Full Text View journal details
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