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PMID: 4874314 Published · ppublish English Journal Article

Effect of mutation to streptomycin resistance on amber suppressor genes.

Journal of bacteriology ·Vol. 96 ·No. 1 ·1968-07-00 ·Pages 43-50

Otsuji N, Aono H

Abstract

Three classes of nonidentical streptomycin-resistant mutations were distinguished in Escherichia coli by their effect on the efficiency of suppression by an amber suppressor gene, sup E. The first class of mutation caused a strong restriction in efficiency of suppression of an amber codon in various cistrons of phage lambda and in an alkaline phosphatase structural gene of E. coli. The second class caused weak restriction, and the third class caused no restriction. The restrictive effect of the streptomycin resistance mutation of the first class on the sup E gene was reduced by addition of streptomycin. This mutation had little effect on efficiencies of suppression by amber suppressor genes sup D and sup F. Analyses on the alkaline phosphatase formed in the suppressor strain indicated that mutation to restrictive streptomycin resistance causes a reduction in translation of the amber codon in the alkaline phosphatase structural gene.

MeSH Terms
Alkaline Phosphatase/analysis,biosynthesis Carbon Isotopes Cell-Free System Chromatography Coliphages Drug Resistance, Microbial Electrophoresis, Disc Escherichia coli/drug effects Genes Genetics, Microbial Lysogeny Molecular Biology Mutation Proline/metabolism Streptomycin/pharmacology Suppression, Genetic
Chemicals
Carbon Isotopes Proline Alkaline Phosphatase Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Otsuji N
Aono H
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.96.1.43-50.1968
Published
1968-07-00
Pages
43-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC252250
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.96.1.43-50.1968 PMC Full Text View journal details
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