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PMID: 10205182 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.

The EMBO journal ·Vol. 18 ·No. 8 ·1999-04-15 ·Pages 2294-303

de Moor CH, Richter JD

Abstract

During oocyte maturation, cyclin B1 mRNA is translationally activated by cytoplasmic polyadenylation. This process is dependent on cytoplasmic polyadenylation elements (CPEs) in the 3' untranslated region (UTR) of the mRNA. To determine whether a titratable factor might be involved in the initial translational repression (masking) of this mRNA, high levels of cyclin B1 3' UTR were injected into oocytes. While this treatment had no effect on the poly(A) tail length of endogenous cyclin B1 mRNA, it induced cyclin B1 synthesis. A mutational analysis revealed that the most efficient unmasking element in the cyclin 3' UTR was the CPE. However, other U-rich sequences that resemble the CPE in structure, but which do not bind the CPE-binding polyadenylation factor CPEB, failed to induce unmasking. When fused to the chloramphenical acetyl transferase (CAT) coding region, the cyclin B1 3' UTR inhibited CAT translation in injected oocytes. In addition, a synthetic 3' UTR containing multiple copies of the CPE also inhibited translation, and did so in a dose-dependent manner. Furthermore, efficient CPE-mediated masking required cap-dependent translation. During the normal course of progesterone-induced maturation, cytoplasmic polyadenylation was necessary for mRNA unmasking. A model to explain how cyclin B1 mRNA masking and unmasking could be regulated by the CPE is presented.

MeSH Terms
3' Untranslated Regions Animals Base Sequence Chloramphenicol O-Acetyltransferase/genetics Cyclin B/genetics Cyclin B1 Cytoplasm/metabolism DNA Primers Oocytes/metabolism Poly A/metabolism Protein Biosynthesis RNA, Messenger/genetics,metabolism Xenopus laevis
Chemicals
3' Untranslated Regions Cyclin B Cyclin B1 DNA Primers RNA, Messenger Poly A Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
de Moor C H
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Richter J D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/18.8.2294
Published
1999-04-15
Pages
2294-303
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171312
Subset
IM
Grants
NIGMS NIH HHS · GM46779 · United States
External Links
PubMed source DOI: 10.1093/emboj/18.8.2294 PMC Full Text View journal details
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