Abstract
The Escherichia coli transcription factor OxyR is activated by the formation of an intramolecular disulfide bond and subsequently is deactivated by enzymatic reduction of the disulfide bond. Here we show that OxyR can be activated by two possible pathways. In mutants defective in the cellular disulfide-reducing systems, OxyR is constitutively activated by a change in the thiol-disulfide redox status in the absence of added oxidants. In wild-type cells, OxyR is activated by hydrogen peroxide. By monitoring the presence of the OxyR disulfide bond after exposure to hydrogen peroxide in vivo and in vitro, we also show that the kinetics of OxyR oxidation by low concentrations of hydrogen peroxide is significantly faster than the kinetics of OxyR reduction, allowing for transient activation in an overall reducing environment. We propose that the activity of OxyR in vivo is determined by the balance between hydrogen peroxide levels and the cellular redox environment.
MeSH Terms
Alkaline Phosphatase/metabolism
Bacterial Proteins/metabolism
DNA-Binding Proteins
Escherichia coli/genetics,metabolism
Escherichia coli Proteins
Genotype
Glutathione/metabolism
Glutathione Disulfide/metabolism
Hydrogen Peroxide/pharmacology
Kinetics
Models, Chemical
Oxidation-Reduction
Repressor Proteins/metabolism
Transcription Factors/metabolism
Chemicals
Bacterial Proteins
DNA-Binding Proteins
Escherichia coli Proteins
Repressor Proteins
Transcription Factors
oxyR protein, E coli
Hydrogen Peroxide
Alkaline Phosphatase
Glutathione
Glutathione Disulfide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aslund F
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
Zheng M
Beckwith J
Storz G
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