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PMID: 10359564 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myeloperoxidase-generated reactive nitrogen species convert LDL into an atherogenic form in vitro.

The Journal of clinical investigation ·Vol. 103 ·No. 11 ·1999-06-00 ·Pages 1547-60

Podrez EA, Schmitt D, Hoff HF, Hazen SL

Abstract

Oxidized LDL is implicated in atherosclerosis; however, the pathways that convert LDL into an atherogenic form in vivo are not established. Production of reactive nitrogen species may be one important pathway, since LDL recovered from human atherosclerotic aorta is enriched in nitrotyrosine. We now report that reactive nitrogen species generated by the MPO-H2O2-NO2- system of monocytes convert LDL into a form (NO2-LDL) that is avidly taken up and degraded by macrophages, leading to massive cholesterol deposition and foam cell formation, essential steps in lesion development. Incubation of LDL with isolated MPO, an H2O2-generating system, and nitrite (NO2-)-- a major end-product of NO metabolism--resulted in nitration of apolipoprotein B 100 tyrosyl residues and initiation of LDL lipid peroxidation. The time course of LDL protein nitration and lipid peroxidation paralleled the acquisition of high-affinity, concentration-dependent, and saturable binding of NO2-LDL to human monocyte-derived macrophages and mouse peritoneal macrophages. LDL modification and conversion into a high-uptake form occurred in the absence of free metal ions, required NO2-, occurred at physiological levels of Cl-, and was inhibited by heme poisons, catalase, and BHT. Macrophage binding of NO2-LDL was specific and mediated by neither the LDL receptor nor the scavenger receptor class A type I. Exposure of macrophages to NO2-LDL promoted cholesteryl ester synthesis, intracellular cholesterol and cholesteryl ester accumulation, and foam cell formation. Collectively, these results identify MPO-generated reactive nitrogen species as a physiologically plausible pathway for converting LDL into an atherogenic form.

MeSH Terms
Animals Arteriosclerosis/metabolism Cholesterol Esters/biosynthesis Humans Hydrogen Peroxide/metabolism Lipid Peroxidation Lipoproteins, LDL/metabolism Macrophages/metabolism Mice Mice, Inbred C57BL Monocytes/metabolism Nitrites/metabolism Nitrogen Dioxide/metabolism Peroxidase/metabolism
Chemicals
Cholesterol Esters Lipoproteins, LDL Nitrites Hydrogen Peroxide Peroxidase Nitrogen Dioxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Podrez E A
Department of Cell Biology, and Department of Cardiology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Schmitt D
Hoff H F
Hazen S L
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
Published
1999-06-00
Pages
1547-60
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC408366
Subset
IM
Grants
NHLBI NIH HHS · R01 HL061878 · United States
NHLBI NIH HHS · R01 HL053315 · United States
NHLBI NIH HHS · HL-53315 · United States
NHLBI NIH HHS · HL-62526 · United States
NHLBI NIH HHS · HL-61878 · United States
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