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PMID: 10359792 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Rpd3 histone deacetylase is required for segmentation of the Drosophila embryo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 12 ·1999-06-08 ·Pages 6797-801

Mannervik M, Levine M

Abstract

Previous studies have implicated histone deacetylation and chromatin condensation as critical mechanisms of transcription repression in yeast and mammals. A specific histone deacetylase, Rpd3, interacts with a variety of sequence-specific transcriptional repressors, including Mad-Max heterodimers and members of the nuclear receptor superfamily. Here, we present evidence that a strong hypomorphic mutation in the Drosophila Rpd3 gene causes embryonic lethality and a specific pair-rule segmentation phenotype. The analysis of a number of segmentation genes suggests that the repressor function of Even-skipped (Eve) may be diminished, causing an indirect loss of Ftz-mediated activation of engrailed. The relatively mild defects observed in Rpd3 mutants suggest that the recently identified Groucho and dCtBP corepressor proteins do not function solely through the recruitment of histone deacetylases. We discuss the possibility that Eve mediates multiple mechanisms of repression, so that Rpd3 mutants disrupt the regulation of just a subset of Eve target genes.

MeSH Terms
Animals Drosophila/embryology,physiology Drosophila Proteins Embryo, Nonmammalian/embryology,physiology Gene Expression Regulation, Developmental/physiology Histone Deacetylase 1 Histone Deacetylases/physiology Mutation Repressor Proteins Transcription Factors/physiology
Chemicals
Drosophila Proteins Repressor Proteins Transcription Factors HDAC1 protein, Drosophila Histone Deacetylase 1 Histone Deacetylases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mannervik M
Department of Molecular and Cellular Biology, Division of Genetics, 401 Barker Hall, University of California, Berkeley, CA 94720, USA.
Levine M
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.12.6797
Published
1999-06-08
Pages
6797-801
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21995
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034431 · United States
NIGMS NIH HHS · GM 34431 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.12.6797 PMC Full Text View journal details
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