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PMID: 10359847 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 12 ·1999-06-08 ·Pages 7110-5

Vatamaniuk OK, Mari S, Lu YP, Rea PA

Abstract

Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.

MeSH Terms
Amino Acid Sequence Aminoacyltransferases/genetics,isolation & purification,metabolism Arabidopsis/enzymology Molecular Sequence Data Plant Proteins/genetics,isolation & purification,metabolism Saccharomyces cerevisiae Sequence Alignment
Chemicals
Plant Proteins Aminoacyltransferases glutathione gamma-glutamylcysteinyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vatamaniuk O K
Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Mari S
Lu Y P
Rea P A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.12.7110
Published
1999-06-08
Pages
7110-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22073
Subset
IM
Databases
GENBANK
AF085230, AF085231
External Links
PubMed source DOI: 10.1073/pnas.96.12.7110 PMC Full Text View journal details
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