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PMID: 10364328 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

La autoantigen specifically recognizes a predicted stem-loop in hepatitis B virus RNA.

Journal of virology ·Vol. 73 ·No. 7 ·1999-07-00 ·Pages 5767-76

Heise T, Guidotti LG, Chisari FV

Abstract

We recently identified three nuclear proteins (p45, p39, and p26) that bind to a 91-nucleotide (nt) RNA element between nt 1243 and 1333 in hepatitis B virus (HBV) RNA, and we showed that these proteins and HBV RNA are regulated coordinately by gamma interferon and tumor necrosis factor alpha. Purification and sequence analysis of tryptic peptides obtained from p39 revealed sequence homology to the mouse La protein. Immunoprecipitation experiments showed that p45, p39, and p26 were recognized by anti-La-specific antiserum, indicating that p45 is the full-length La protein and that p39 and p26 are likely to be proteolytic La cleavage products. Furthermore, in competition experiments we found that all three La proteins bind, in a phosphorylation-dependent manner, to the same predicted stem-loop structure located between nt 1275 and 1291 of HBV, with Kds of approximately 1.0 nM. Collectively, these results support the notion that the La protein may contribute to HBV RNA stability, constitutively and in response to inflammatory cytokines.

MeSH Terms
Amino Acid Sequence Animals Autoantigens/metabolism Base Sequence Cell Nucleus/metabolism Hepatitis B virus/genetics Humans Male Mice Mice, Transgenic Molecular Sequence Data Nucleic Acid Conformation Phosphorylation RNA, Viral/chemistry,metabolism RNA-Binding Proteins/metabolism Ribonucleoproteins/metabolism
Chemicals
Autoantigens RNA, Viral RNA-Binding Proteins Ribonucleoproteins SS-B antigen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heise T
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA.
Guidotti L G
Chisari F V
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.7.5767-5776.1999
Published
1999-07-00
Pages
5767-76
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112637
Subset
IM
Grants
NIAID NIH HHS · R01 AI040696 · United States
NCI NIH HHS · R37 CA040489 · United States
NIAID NIH HHS · R01-AI40696 · United States
NCI NIH HHS · R37-CA40489 · United States
External Links
PubMed source DOI: 10.1128/JVI.73.7.5767-5776.1999 PMC Full Text View journal details
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