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PMID: 10398681 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana.

Genes & development ·Vol. 13 ·No. 13 ·1999-07-01 ·Pages 1678-91

Gray WM, del Pozo JC, Walker L, Hobbie L, Risseeuw E, Banks T, Crosby WL, Yang M, Ma H, Estelle M

Abstract

The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCFTIR1. The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCFTIR1 is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.

MeSH Terms
Amino Acid Sequence Arabidopsis/drug effects,enzymology,genetics,growth & development Arabidopsis Proteins Gene Expression Regulation, Plant Indoleacetic Acids/pharmacology Molecular Sequence Data Peptide Synthases/genetics,physiology Plant Proteins/genetics,physiology Plant Roots/growth & development Recombinant Fusion Proteins/physiology SKP Cullin F-Box Protein Ligases Sequence Alignment
Chemicals
ASK1 protein, Arabidopsis Arabidopsis Proteins Indoleacetic Acids Plant Proteins Recombinant Fusion Proteins indoleacetic acid ASK2 protein, Arabidopsis SKP Cullin F-Box Protein Ligases Peptide Synthases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Gray W M
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
del Pozo J C
Walker L
Hobbie L
Risseeuw E
Banks T
Crosby W L
Yang M
Ma H
Estelle M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.13.1678
Published
1999-07-01
Pages
1678-91
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316846
Subset
IM
Grants
NIGMS NIH HHS · F32 GM018680 · United States
NIGMS NIH HHS · R01 GM043644 · United States
NIGMS NIH HHS · GM 18680 · United States
NIGMS NIH HHS · GM43644 · United States
External Links
PubMed source DOI: 10.1101/gad.13.13.1678 PMC Full Text View journal details
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