Home LiteratureArticle Details
PMID: 10427086 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ERG30, a VAP-33-related protein, functions in protein transport mediated by COPI vesicles.

The Journal of cell biology ·Vol. 146 ·No. 2 ·1999-07-26 ·Pages 301-11

Soussan L, Burakov D, Daniels MP, Toister-Achituv M, Porat A, Yarden Y, Elazar Z

Abstract

Intracellular transport of newly synthesized and mature proteins via vesicles is controlled by a large group of proteins. Here we describe a ubiquitous rat protein-endoplasmic reticulum (ER) and Golgi 30-kD protein (ERG30)-which shares structural characteristics with VAP-33, a 33-kD protein from Aplysia californica which was shown to interact with the synaptic protein VAMP. The transmembrane topology of the 30-kD ERG30 corresponds to a type II integral membrane protein, whose cytoplasmic NH(2) terminus contains a predicted coiled-coil motif. We localized ERG30 to the ER and to pre-Golgi intermediates by biochemical and immunocytochemical methods. Consistent with a role in vesicular transport, anti-ERG30 antibodies specifically inhibit intra-Golgi transport in vitro, leading to significant accumulation of COPI-coated vesicles. It appears that ERG30 functions early in the secretory pathway, probably within the Golgi and between the Golgi and the ER.

MeSH Terms
Amino Acid Sequence Animals Antibodies/pharmacology Base Sequence Biological Transport/drug effects Carrier Proteins/chemistry,genetics,immunology,metabolism Cell Line Cloning, Molecular Coated Vesicles/drug effects,metabolism Coatomer Protein Endoplasmic Reticulum, Rough/metabolism Gene Expression Golgi Apparatus/drug effects,metabolism Membrane Proteins/chemistry,genetics,immunology,metabolism,physiology Molecular Sequence Data Protein Binding Protein Structure, Secondary Rats SNARE Proteins Sequence Deletion Sequence Homology, Amino Acid Vesicular Transport Proteins Yeasts/genetics
Chemicals
Antibodies Carrier Proteins Coatomer Protein ERG30 protein, rat Membrane Proteins SNARE Proteins VAPA protein, human Vapa protein, rat Vesicular Transport Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Soussan L
Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, 76100 Israel.
Burakov D
Daniels M P
Toister-Achituv M
Porat A
Yarden Y
Elazar Z
References (50)
50 references, click to expand
  1. Coatomer interaction with di-lysine endoplasmic reticulum retention motifs.
    Science. 1994 Mar 18;263(5153):1629-31 PMID: 8128252
  2. Ca2+/calmodulin signals the completion of docking and triggers a late step of vacuole fusion.
    Nature. 1998 Dec 10;396(6711):575-80 PMID: 9859992
  3. Mechanisms of intracellular protein transport.
    Nature. 1994 Nov 3;372(6501):55-63 PMID: 7969419
  4. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.
    Cell. 1994 Dec 30;79(7):1199-207 PMID: 8001155
  5. Biosynthetic processing of neu differentiation factor. Glycosylation trafficking, and regulated cleavage from the cell surface.
    J Biol Chem. 1995 Aug 11;270(32):19188-96 PMID: 7642587
  6. An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):8011-5 PMID: 7644530
  7. A VAMP-binding protein from Aplysia required for neurotransmitter release.
    Science. 1995 Sep 15;269(5230):1580-3 PMID: 7667638
  8. Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae.
    J Biochem. 1995 Jul;118(1):39-45 PMID: 8537323
  9. Reconstitution in vitro of the motile apparatus from the amoeboid sperm of Ascaris shows that filament assembly and bundling move membranes.
    Cell. 1996 Jan 12;84(1):105-14 PMID: 8548814
  10. Coat proteins and vesicle budding.
    Science. 1996 Mar 15;271(5255):1526-33 PMID: 8599108
  11. Assembly of the ER to Golgi SNARE complex requires Uso1p.
    J Cell Biol. 1996 Mar;132(5):755-67 PMID: 8603910
  12. Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles.
    Cell. 1996 Apr 5;85(1):83-94 PMID: 8620540
  13. Proteins functioning in synaptic transmission at the sensory to motor synapse of Aplysia.
    Neuropharmacology. 1995 Nov;34(11):1379-85 PMID: 8606787
  14. Budding vesicles in living cells.
    Sci Am. 1996 Mar;274(3):70-5 PMID: 8607018
  15. Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes.
    J Cell Biol. 1996 Feb;132(3):277-89 PMID: 8636207
  16. The organization of endoplasmic reticulum export complexes.
    J Cell Biol. 1996 Oct;135(1):19-35 PMID: 8858160
  17. Transport vesicle docking: SNAREs and associates.
    Annu Rev Cell Dev Biol. 1996;12:441-61 PMID: 8970734
  18. Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures.
    J Histochem Cytochem. 1996 Dec;44(12):1481-8 PMID: 8985140
  19. Selective packaging of cargo molecules into endoplasmic reticulum-derived COPII vesicles.
    Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):837-42 PMID: 9023343
  20. A conserved domain is present in different families of vesicular fusion proteins: a new superfamily.
    Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3046-51 PMID: 9096343
  21. The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.
    Cell. 1997 May 2;89(3):445-55 PMID: 9150144
  22. t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase.
    Science. 1997 May 23;276(5316):1255-8 PMID: 9157884
  23. Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin, and SNAP-25 in the membrane of synaptic vesicles.
    Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6197-201 PMID: 9177194
  24. Bidirectional transport by distinct populations of COPI-coated vesicles.
    Cell. 1997 Jul 25;90(2):335-49 PMID: 9244307
  25. Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy.
    Cell. 1997 Aug 8;90(3):523-35 PMID: 9267032
  26. Osmotic stress activated expression of an Arabidopsis plasma membrane-associated protein: sequence and predicted secondary structure.
    Biochim Biophys Acta. 1997 Aug 15;1341(1):79-86 PMID: 9300811
  27. Characterization of the Golgi complex cleared of proteins in transit and examination of calcium uptake activities.
    Mol Biol Cell. 1997 Oct;8(10):1911-31 PMID: 9348533
  28. Structural changes are associated with soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor complex formation.
    J Biol Chem. 1997 Oct 31;272(44):28036-41 PMID: 9346956
  29. A role for giantin in docking COPI vesicles to Golgi membranes.
    J Cell Biol. 1998 Mar 9;140(5):1013-21 PMID: 9490716
  30. SNAREpins: minimal machinery for membrane fusion.
    Cell. 1998 Mar 20;92(6):759-72 PMID: 9529252
  31. The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism.
    J Bacteriol. 1998 Apr;180(7):1700-8 PMID: 9537365
  32. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  33. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  34. Isolation and characterization of a sperm-specific gene family in the nematode Caenorhabditis elegans.
    Mol Cell Biol. 1984 Mar;4(3):529-37 PMID: 6325882
  35. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
    Cell. 1984 Dec;39(2 Pt 1):405-16 PMID: 6498939
  36. Development of ultrastructural specializations during the formation of acetylcholine receptor aggregates on cultured myotubes.
    J Neurosci. 1986 Feb;6(2):487-97 PMID: 3512791
  37. Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
    Cell. 1989 Mar 10;56(5):801-13 PMID: 2647301
  38. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    Cell. 1989 Jul 28;58(2):329-36 PMID: 2752426
  39. A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack.
    J Cell Biol. 1992 Sep;118(5):1015-26 PMID: 1512287
  40. SNAP receptors implicated in vesicle targeting and fusion.
    Nature. 1993 Mar 25;362(6418):318-24 PMID: 8455717
  41. Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
    J Biol Chem. 1993 Jun 5;268(16):12083-9 PMID: 8505331
  42. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion.
    Cell. 1993 Nov 5;75(3):409-18 PMID: 8221884
  43. Stepwise assembly of functionally active transport vesicles.
    Cell. 1993 Dec 3;75(5):1015-25 PMID: 8252615
  44. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins.
    EMBO J. 1998 Apr 15;17(8):2156-65 PMID: 9545229
  45. Identification of a human homologue of the vesicle-associated membrane protein (VAMP)-associated protein of 33 kDa (VAP-33): a broadly expressed protein that binds to VAMP.
    Biochem J. 1998 Jul 15;333 ( Pt 2):247-51 PMID: 9657962
  46. Getting into the Golgi.
    Trends Cell Biol. 1998 Jan;8(1):21-5 PMID: 9695803
  47. A model for structural similarity between different SNARE complexes based on sequence relationships.
    Trends Cell Biol. 1998 Jul;8(7):260-2 PMID: 9714596
  48. Purification and characterization of a novel 13 S hetero-oligomeric protein complex that stimulates in vitro Golgi transport.
    J Biol Chem. 1998 Nov 6;273(45):29565-76 PMID: 9792665
  49. Defining the functions of trans-SNARE pairs.
    Nature. 1998 Dec 10;396(6711):543-8 PMID: 9859990
  50. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.
    Cell. 1994 Jun 17;77(6):895-907 PMID: 8004676
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.146.2.301
Published
1999-07-26
Pages
301-11
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2156184
Subset
IM
Grants
NCI NIH HHS · R01 CA072981 · United States
NCI NIH HHS · R37 CA072981 · United States
NCI NIH HHS · CA 72981 · United States
External Links
PubMed source DOI: 10.1083/jcb.146.2.301 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2