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PMID: 7644530 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 17 ·1995-08-15 ·Pages 8011-5

Stamnes MA, Craighead MW, Hoe MH, Lampen N, Geromanos S, Tempst P, Rothman JE

Abstract

We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.

MeSH Terms
ADP-Ribosylation Factors Amino Acid Sequence Animals Arabidopsis/metabolism Base Sequence Brain/metabolism CHO Cells Carrier Proteins/metabolism Cattle Coatomer Protein Cricetinae Cytosol/metabolism DNA Primers Dogs GTP-Binding Proteins/metabolism Golgi Apparatus/metabolism,ultrastructure Humans Membrane Fusion Membrane Proteins/biosynthesis,chemistry,metabolism Microscopy, Electron Molecular Sequence Data Organelles/physiology Polymerase Chain Reaction Saccharomyces cerevisiae/physiology Sequence Homology, Amino Acid
Chemicals
Carrier Proteins Coatomer Protein DNA Primers Membrane Proteins GTP-Binding Proteins ADP-Ribosylation Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Stamnes M A
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Craighead M W
Hoe M H
Lampen N
Geromanos S
Tempst P
Rothman J E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.17.8011
Published
1995-08-15
Pages
8011-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41276
Subset
IM
Databases
GENBANK
U26264, X67317
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.92.17.8011 PMC Full Text View journal details
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