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PMID: 10428945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Contribution of kasA analysis to detection of isoniazid-resistant Mycobacterium tuberculosis in Singapore.

Antimicrobial agents and chemotherapy ·Vol. 43 ·No. 8 ·1999-08-00 ·Pages 2087-9

Lee AS, Lim IH, Tang LL, Telenti A, Wong SY

Abstract

Genotypic analysis of resistance to isoniazid (INH) in Mycobacterium tuberculosis is complex due to the various genes potentially involved. Mutations in ketoacyl acyl carrier protein synthase (encoded by kasA) were present in 16 of 160 (10%) INH-resistant isolates (R121K [n = 1], G269S [n = 3], G312S [n = 11], G387D [n = 1]). However, G312S was also present in 6 of 32 (19%) susceptible strains. kasA analysis contributed marginally to the performance of INH genotypic testing in Singapore. The significance of kasA polymorphisms in INH resistance should be carefully established.

MeSH Terms
3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics Antitubercular Agents/pharmacology Drug Resistance, Microbial/genetics Genotype Isoniazid/pharmacology Mutation Mycobacterium tuberculosis/drug effects,genetics Singapore
Chemicals
Antitubercular Agents 3-ketoacyl-acyl carrier protein synthase III 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase Isoniazid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee A S
Department of Clinical Research, Ministry of Health, Singapore General Hospital, Singapore 169608. [email protected]
Lim I H
Tang L L
Telenti A
Wong S Y
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.43.8.2087
Email
[email protected]
Published
1999-08-00
Pages
2087-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC89423
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.43.8.2087 PMC Full Text View journal details
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