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PMID: 10432293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides.

The Biochemical journal ·Vol. 342 ( Pt 1) ·1999-08-15 ·Pages 7-12

Dowler S, Currie RA, Downes CP, Alessi DR

Abstract

We have identified a novel 280 amino acid protein which contains a putative myristoylation site at its N-terminus followed by an Src homology (SH2) domain and a pleckstrin homology (PH) domain at its C-terminus. It has been termed dual adaptor for phosphotyrosine and 3-phosphoinositides (DAPP1). DAPP1 is widely expressed and exhibits high-affinity interactions with PtdIns(3,4,5)P(3) and PtdIns(3,4)P(2), but not with other phospholipids tested. These observations predict that DAPP1 will interact with both tyrosine phosphorylated proteins and 3-phosphoinositides and may therefore play a role in regulating the location and/or activity of such proteins(s) in response to agonists that elevate PtdIns(3,4,5)P(3) and PtdIns(3,4)P(2).

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Base Sequence Blood Proteins/chemistry,genetics,metabolism Carrier Proteins/chemistry,genetics,metabolism Cell Line Cloning, Molecular Expressed Sequence Tags Fatty Acids Humans Lipoproteins Mice Molecular Sequence Data Phosphatidylinositol 3-Kinases/metabolism Phosphatidylinositol Phosphates/chemistry,metabolism Phosphotyrosine/metabolism RNA, Messenger/analysis,genetics Receptor Protein-Tyrosine Kinases/metabolism Recombinant Fusion Proteins/chemistry,genetics,metabolism Sequence Deletion Sequence Homology, Amino Acid Signal Transduction Stereoisomerism src Homology Domains/genetics,physiology
Chemicals
Adaptor Proteins, Signal Transducing Blood Proteins Carrier Proteins DAPP1 protein, human Dapp1 protein, mouse Fatty Acids Lipoproteins Phosphatidylinositol Phosphates RNA, Messenger Recombinant Fusion Proteins phosphatidylinositol 3,4,5-triphosphate phosphatidylinositol 3,4-diphosphate Phosphotyrosine Phosphatidylinositol 3-Kinases Receptor Protein-Tyrosine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dowler S
MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI/WTB Complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U.K. [email protected]
Currie R A
Downes C P
Alessi D R
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Email
[email protected]
Published
1999-08-15
Pages
7-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1220429
Subset
IM
Databases
GENBANK
AF163254, AF163255
External Links
PubMed source PMC Full Text View journal details
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