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PMID: 2841581 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The first seven amino acids encoded by the v-src oncogene act as a myristylation signal: lysine 7 is a critical determinant.

Molecular and cellular biology ·Vol. 8 ·No. 6 ·1988-06-00 ·Pages 2435-41

Kaplan JM, Mardon G, Bishop JM, Varmus HE

Abstract

The transforming protein of Rous sarcoma virus, pp60v-src, is covalently coupled to myristic acid by an amide linkage to glycine 2. Myristylation promotes the association of pp60v-src with cellular membranes, and this subcellular location is essential for transforming activity. The findings presented here, in conjunction with the previous reports of others, imply that the seventh amino acid encoded by v-src might be important in the myristylation reaction. Replacement of lysine 7 by asparagine greatly reduced the myristylation, membrane association, and transforming activity of pp60v-src. In contrast, substitution of arginine at residue 7 had no effect on any of these properties of pp60v-src. Addition of amino acids 1 to 7 encoded by v-src was sufficient to cause myristylation of a src-pyruvate kinase fusion protein. We conclude that the recognition sequence for myristylation of pp60v-src comprises amino acids 1 to 7 and that lysine 7 is a critical component of this sequence.

MeSH Terms
Acylation Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Avian Sarcoma Viruses/genetics Cells, Cultured Chick Embryo Electrophoresis, Polyacrylamide Gel Fibroblasts/microbiology Lysine/analysis Myristic Acid Myristic Acids/metabolism Oncogene Protein pp60(v-src) Protein Kinases/analysis,metabolism Retroviridae Proteins/analysis,immunology,metabolism
Chemicals
Antibodies, Monoclonal Myristic Acids Retroviridae Proteins Myristic Acid Protein Kinases Oncogene Protein pp60(v-src) Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaplan J M
Department of Microbiology, University of California, San Francisco 94143.
Mardon G
Bishop J M
Varmus H E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.6.2435-2441.1988
Published
1988-06-00
Pages
2435-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363442
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.6.2435-2441.1988 PMC Full Text View journal details
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