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PMID: 3553615 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the feline c-fes/fps proto-oncogene: genesis of a retroviral oncogene.

Journal of virology ·Vol. 61 ·No. 6 ·1987-06-00 ·Pages 2009-16

Roebroek AJ, Schalken JA, Onnekink C, Bloemers HP, Van de Ven WJ

Abstract

The nucleotide sequence of the feline c-fes/fps proto-oncogene was analyzed. Comparison with v-fes and v-fps revealed that all v-fes/fps homologous sequences were dispersed over 11 kilobase pairs in 19 interspersed segments. All segments, numbered exon 1 to exon 19 as in the chicken and human loci, were flanked by consensus splice junctions. The putative promoter region contained a CATT sequence and three CCGCCC motifs which were also found in the human locus at similar positions. About 200 nucleotides downstream of a translational stop codon in exon 19, a putative poly(A) addition signal was identified. Using the putative translation initiation codon in exon 2, a 93,000-molecular-weight protein could be deduced. This protein resembled very well the putative protein of the human c-fes/fps proto-oncogene (94% overall homology) and, although less well, the putative protein of the chicken c-fes/fps proto-oncogene (70% overall homology). As far as the feline c-fes/fps proto-oncogene sequences transduced to the Gardner-Arnstein (GA) and Snyder-Theilen (ST) strains of feline sarcoma virus (FeSV) are concerned, homology in deduced amino acid sequences between the GA- and ST-v-fes viral oncogenes and the proto-oncogene was 99%. Analysis of the recombination junctions between feline leukemia virus and v-fes sequences in GA- and ST-FeSV proviral DNA revealed for the left-hand junction the involvement of homologous recombination, presumably at the DNA level. The right-hand junction, which appeared identical in the GA-FeSV and ST-FeSV genomes, could have been the result of a site-specific recombination at the RNA level.

MeSH Terms
Animals Base Sequence Cats/genetics Chickens/genetics Gene Products, gag Genes Genes, Viral Humans Models, Genetic Oncogenes Protein-Tyrosine Kinases Proto-Oncogene Mas Proto-Oncogene Proteins/genetics Proto-Oncogenes Recombination, Genetic Retroviridae Proteins/genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Gene Products, gag MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins Retroviridae Proteins proto-oncogene protein c-fes-fps Protein-Tyrosine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roebroek A J
Schalken J A
Onnekink C
Bloemers H P
Van de Ven W J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.6.2009-2016.1987
Published
1987-06-00
Pages
2009-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254210
Subset
IM
Databases
GENBANK
M16665, M16666, M16667, M16668, M16669, M16670, M16671, M16672, M16673, M16674, M16698, M16699, M16700, M16701, M16702, M16703, M16704, M16705, M16706
External Links
PubMed source DOI: 10.1128/JVI.61.6.2009-2016.1987 PMC Full Text View journal details
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