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PMID: 6254060 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5158-62

Barbacid M, Beemon K, Devare SG

Abstract

The only known product of the Snyder-Theilen strain of feline sarcoma virus (ST-FeSV) is a 85,000-dalton protein, designated ST P85, that contains feline leukemia virus gag gene encoded proteins (p15, p12, and a fragment of p30) and a sarcoma virus-specific polypeptide. Antibodies directed against the latter immunoprecipitated a 92,000-dalton phosphoprotein (NCP 92) expressed at low levels in normal feline embryo fibroblasts as well as in feline cells of epithelial or lymphoid origin. Normal cellular proteins crossreactive with ST P85 were also detected in cell lines from various other mammalian species. These results suggest that the ST-FeSV sequences encoding for the sarcoma virus-specific domain of ST P85 originated from an evolutionarily conserved cellular gene expressed in cells of independent differentiation lineage. Immunoprecipitates containing ST-FeSV P85 exhibited a protein kinase activity that specifically phosphorylated tyrosine residues. The physiological significance of this finding is illustrated by the finding that phosphotyrosine is an intrinsic component of ST P85. Furthermore, 5- to-fold higher levels of this unusual phosphorylated amino acid were present in ST-FeSV transformants than in uninfected control cells. Phosphorylation of tyrosine residues appears to be associated with cellular transformation caused by Rous sarcoma virus and Abelson murine leukemia virus. Thus, independent transforming virus isolates from birds, mice, and cats may utilize common pathways in exerting their oncogenic potential.

MeSH Terms
Animals Cats Gene Products, gag Mammals/genetics Oncogenic Viruses/enzymology Phosphorylation Protein Kinases/genetics,physiology Protein Precursors/genetics Rats Retroviridae/genetics Sarcoma Viruses, Feline/genetics,metabolism Tyrosine/metabolism Viral Proteins/genetics,immunology,physiology
Chemicals
Gene Products, gag Protein Precursors Viral Proteins Tyrosine Protein Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barbacid M
Beemon K
Devare S G
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.9.5158
Published
1980-09-00
Pages
5158-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350016
Subset
IM
Grants
NCI NIH HHS · CA23896 · United States
NCI NIH HHS · N0L-CP-61024 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.9.5158 PMC Full Text View journal details
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