Home LiteratureArticle Details

PMID: 10438871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intracellular distribution of rubella virus nonstructural protein P150.

Journal of virology ·Vol. 73 ·No. 9 ·1999-09-00 ·Pages 7805-11

Kujala P, Ahola T, Ehsani N, Auvinen P, Vihinen H, Kääriäinen L

Abstract

Antiserum prepared against an amino-terminal fragment of rubella virus (RUB) nonstructural polyprotein was used to study RUB-infected Vero cells. Replicase protein P150 was associated with vesicles and vacuoles of endolysosomal origin and later with large, convoluted, tubular membrane structures. Newly incorporated bromouridine was associated with the same structures and specifically with small membrane invaginations, spherules, indicating that these structures may be the sites of viral RNA synthesis.

MeSH Terms

Animals Chlorocebus aethiops HeLa Cells Humans Intracellular Fluid Microscopy, Confocal RNA, Viral/biosynthesis RNA-Dependent RNA Polymerase/metabolism Rabbits Rubella virus/enzymology Vero Cells Viral Nonstructural Proteins/metabolism

Chemicals

RNA, Viral Viral Nonstructural Proteins RNA-Dependent RNA Polymerase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kujala P

Institute of Biotechnology, University of Helsinki, Helsinki, Finland.

Ahola T

Ehsani N

Auvinen P

Vihinen H

Kääriäinen L

References (31)

31 references, click to expand

Replication of Semliki Forest virus: an electron microscopic study.
Virology. 1967 May;32(1):128-43 PMID: 6067298
Molecular aspects of the endocytic pathway.
Biochem J. 1998 Dec 1;336 ( Pt 2):271-82 PMID: 9820800
Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication.
J Virol. 1967 Apr;1(2):374-83 PMID: 5630381
Specific membranous structures associated with the replication of group A arboviruses.
J Virol. 1972 Sep;10(3):492-503 PMID: 4672392
Membrane-associated replication complex in arbovirus infection.
J Virol. 1972 Sep;10(3):504-15 PMID: 4342056
Rubella virus contains one capsid protein and three envelope glycoproteins, E1, E2a, and E2b.
J Virol. 1983 Jun;46(3):964-73 PMID: 6854740
The gene order for rubella virus structural proteins is NH2-C-E2-E1-COOH.
J Virol. 1984 Aug;51(2):354-8 PMID: 6748161
A new method of preparing gold probes for multiple-labeling cytochemistry.
Eur J Cell Biol. 1985 Jul;38(1):87-93 PMID: 4029177
Distribution by immunofluorescence of viral products and actin-containing cytoskeletal filaments in rubella virus-infected cells.
Arch Virol. 1987;92(3-4):211-9 PMID: 3545151
Time course of virus-specific macromolecular synthesis during rubella virus infection in Vero cells.
Virology. 1988 Jan;162(1):65-75 PMID: 3336944
Semliki Forest virus-specific non-structural protein nsP3 is a phosphoprotein.
J Gen Virol. 1988 Sep;69 ( Pt 9):2165-78 PMID: 2970523
Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.
J Cell Biol. 1988 Dec;107(6 Pt 1):2075-86 PMID: 2904446
Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy.
Histochem J. 1989 Mar;21(3):163-71 PMID: 2722561
Rubella virus products and their distribution in infected cells.
Subcell Biochem. 1989;15:203-31 PMID: 2678616
Biogenesis of type I cytopathic vacuoles in Semliki Forest virus-infected BHK cells.
J Virol. 1991 Mar;65(3):1623-7 PMID: 1825342
Monoclonal antibody-defined epitope map of expressed rubella virus protein domains.
J Virol. 1991 Aug;65(8):3986-94 PMID: 1712855
Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences.
Crit Rev Biochem Mol Biol. 1993;28(5):375-430 PMID: 8269709
Expression of the rubella virus nonstructural protein ORF and demonstration of proteolytic processing.
Virology. 1994 Feb;198(2):586-92 PMID: 8291241
Characterization of rubella virus replication complexes using antibodies to double-stranded RNA.
Virology. 1994 Apr;200(1):307-12 PMID: 8128633
The alphaviruses: gene expression, replication, and evolution.
Microbiol Rev. 1994 Sep;58(3):491-562 PMID: 7968923
Molecular biology of rubella virus.
Adv Virus Res. 1994;44:69-160 PMID: 7817880
Identification of the rubella virus nonstructural proteins.
Virology. 1995 Feb 1;206(2):843-53 PMID: 7856097
The alphavirus replicase protein nsP1 is membrane-associated and has affinity to endocytic organelles.
Virology. 1995 Apr 20;208(2):610-20 PMID: 7747433
Membrane transport in the endocytic pathway.
Curr Opin Cell Biol. 1995 Aug;7(4):552-63 PMID: 7495576
Characterization of the rubella virus nonstructural protease domain and its cleavage site.
J Virol. 1996 Jul;70(7):4707-13 PMID: 8676497
T7 vectors with modified T7lac promoter for expression of proteins in Escherichia coli.
Anal Biochem. 1996 May 1;236(2):371-3 PMID: 8660525
Isolation and characterization of early endosomes, late endosomes and terminal lysosomes: their role in protein degradation.
J Cell Sci. 1996 Dec;109 ( Pt 12):2905-14 PMID: 9013338
Rubella virus replication complexes are virus-modified lysosomes.
Virology. 1998 Jan 5;240(1):57-63 PMID: 9448689
The rubella virus nonstructural protease requires divalent cations for activity and functions in trans.
J Virol. 1998 May;72(5):4463-6 PMID: 9557742
Alphavirus replicase protein NSP1 induces filopodia and rearrangement of actin filaments.
J Virol. 1998 Dec;72(12):10265-9 PMID: 9811773
Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis.
J Virol. 1968 Nov;2(11):1326-38 PMID: 5750316

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.73.9.7805-7811.1999

Published

1999-09-00

Pages

7805-11

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC104308

Subset

External Links

PubMed source DOI: 10.1128/JVI.73.9.7805-7811.1999 PMC Full Text View journal details

Analysis Services

Intracellular distribution of rubella virus nonstructural protein P150.

Contact

WeChat Official Account