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PMID: 10444600 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Wnt pathway components orient a mitotic spindle in the early Caenorhabditis elegans embryo without requiring gene transcription in the responding cell.

Genes & development ·Vol. 13 ·No. 15 ·1999-08-01 ·Pages 2028-38

Schlesinger A, Shelton CA, Maloof JN, Meneghini M, Bowerman B

Abstract

In a four-cell-stage Caenorhabditis elegans embryo, Wnt signaling polarizes an endoderm precursor called EMS. The polarization of this cell orients its mitotic spindle in addition to inducing endodermal fate in one daughter cell. Reducing the function of Wnt pathway genes, including a newly identified GSK-3beta homolog called gsk-3, disrupts endoderm induction, whereas only a subset of these genes is required for proper EMS mitotic spindle orientation. Wnt pathway genes thought to act downstream of gsk-3 appear not to be required for spindle orientation, suggesting that gsk-3 represents a branch point in the control of endoderm induction and spindle orientation. Orientation of the mitotic spindle does not require gene transcription in EMS, suggesting that Wnt signaling may directly target the cytoskeleton in a responding cell.

MeSH Terms
Amino Acid Sequence Animals Blastomeres/cytology,metabolism Caenorhabditis elegans/cytology,embryology,genetics,metabolism Calcium-Calmodulin-Dependent Protein Kinases/chemistry,genetics,metabolism Cell Lineage Cell Polarity Cells, Cultured Cytoskeleton/metabolism Embryo, Nonmammalian/cytology,metabolism Embryonic Development Endoderm/cytology Genes, Helminth/genetics,physiology Glycogen Synthase Kinase 3 Helminth Proteins/chemistry,genetics,metabolism Molecular Sequence Data Mutation Proto-Oncogene Proteins/genetics,physiology Sequence Homology, Amino Acid Signal Transduction Spindle Apparatus/metabolism Stem Cells/cytology,metabolism Transcription, Genetic/genetics Wnt Proteins Zebrafish Proteins
Chemicals
Helminth Proteins Proto-Oncogene Proteins Wnt Proteins Zebrafish Proteins Calcium-Calmodulin-Dependent Protein Kinases Glycogen Synthase Kinase 3
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schlesinger A
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA.
Shelton C A
Maloof J N
Meneghini M
Bowerman B
References (45)
45 references, click to expand
  1. The genetics of Caenorhabditis elegans.
    Genetics. 1974 May;77(1):71-94 PMID: 4366476
  2. Asymmetric cell division: from A to Z.
    Genes Dev. 1998 Dec 1;12(23):3625-38 PMID: 9851969
  3. The embryonic cell lineage of the nematode Caenorhabditis elegans.
    Dev Biol. 1983 Nov;100(1):64-119 PMID: 6684600
  4. Cellular interactions in early C. elegans embryos.
    Cell. 1987 Jan 30;48(2):241-50 PMID: 3802194
  5. Reversal of cellular polarity and early cell-cell interaction in the embryos of Caenorhabditis elegans.
    Dev Biol. 1987 Aug;122(2):452-63 PMID: 3596018
  6. Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans.
    J Cell Biol. 1987 Nov;105(5):2123-35 PMID: 3680373
  7. Centrosome movement in the early divisions of Caenorhabditis elegans: a cortical site determining centrosome position.
    J Cell Biol. 1989 Sep;109(3):1185-93 PMID: 2768338
  8. Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II.
    Mol Cell Biol. 1989 Oct;9(10):4119-30 PMID: 2586513
  9. Pharyngeal pumping continues after laser killing of the pharyngeal nervous system of C. elegans.
    Neuron. 1989 Oct;3(4):473-85 PMID: 2642006
  10. Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity.
    Cell. 1991 Feb 8;64(3):573-84 PMID: 1846781
  11. skn-1, a maternally expressed gene required to specify the fate of ventral blastomeres in the early C. elegans embryo.
    Cell. 1992 Mar 20;68(6):1061-75 PMID: 1547503
  12. Induction of gut in Caenorhabditis elegans embryos.
    Nature. 1992 May 21;357(6375):255-7 PMID: 1589023
  13. wingless signaling acts through zeste-white 3, the Drosophila homolog of glycogen synthase kinase-3, to regulate engrailed and establish cell fate.
    Cell. 1992 Dec 24;71(7):1167-79 PMID: 1335365
  14. Tissue polarity genes of Drosophila regulate the subcellular location for prehair initiation in pupal wing cells.
    J Cell Biol. 1993 Oct;123(1):209-21 PMID: 8408199
  15. Establishment of gut fate in the E lineage of C. elegans: the roles of lineage-dependent mechanisms and cell interactions.
    Development. 1993 Aug;118(4):1267-77 PMID: 8269853
  16. Early transcription in Caenorhabditis elegans embryos.
    Development. 1994 Feb;120(2):443-51 PMID: 7512022
  17. IME1 gene encodes a transcription factor which is required to induce meiosis in Saccharomyces cerevisiae.
    Dev Genet. 1994;15(2):139-47 PMID: 8205723
  18. Cell polarity in early C. elegans development.
    Dev Suppl. 1993;:279-87 PMID: 8049483
  19. Transient localized accumulation of actin in Caenorhabditis elegans blastomeres with oriented asymmetric divisions.
    Development. 1994 Aug;120(8):2317-28 PMID: 7925032
  20. UME6 is a key regulator of nitrogen repression and meiotic development.
    Genes Dev. 1994 Apr 1;8(7):796-810 PMID: 7926768
  21. Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos.
    Nature. 1995 Apr 13;374(6523):617-22 PMID: 7715701
  22. An analysis of the response to gut induction in the C. elegans embryo.
    Development. 1995 Apr;121(4):1227-36 PMID: 7743934
  23. Cell contacts orient some cell division axes in the Caenorhabditis elegans embryo.
    J Cell Biol. 1995 May;129(4):1071-80 PMID: 7744956
  24. Soma-germline asymmetry in the distributions of embryonic RNAs in Caenorhabditis elegans.
    Development. 1994 Oct;120(10):2823-34 PMID: 7607073
  25. pop-1 encodes an HMG box protein required for the specification of a mesoderm precursor in early C. elegans embryos.
    Cell. 1995 Nov 17;83(4):599-609 PMID: 7585963
  26. Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1.
    Mol Cell Biol. 1996 May;16(5):2518-26 PMID: 8628320
  27. Binding of GSK3beta to the APC-beta-catenin complex and regulation of complex assembly.
    Science. 1996 May 17;272(5264):1023-6 PMID: 8638126
  28. Time-dependent responses to glp-1-mediated inductions in early C. elegans embryos.
    Development. 1996 Jul;122(7):2043-50 PMID: 8681785
  29. Repression of gene expression in the embryonic germ lineage of C. elegans.
    Nature. 1996 Aug 22;382(6593):713-6 PMID: 8751441
  30. Onset of C. elegans gastrulation is blocked by inhibition of embryonic transcription with an RNA polymerase antisense RNA.
    Dev Biol. 1996 Sep 15;178(2):472-83 PMID: 8812143
  31. Chromophore formation in green fluorescent protein.
    Biochemistry. 1997 Jun 3;36(22):6786-91 PMID: 9184161
  32. Overexpression of the mouse dishevelled-1 protein inhibits GSK-3beta-mediated phosphorylation of tau in transfected mammalian cells.
    FEBS Lett. 1997 Jul 14;411(2-3):369-72 PMID: 9271238
  33. Wnt signaling polarizes an early C. elegans blastomere to distinguish endoderm from mesoderm.
    Cell. 1997 Aug 22;90(4):695-705 PMID: 9288749
  34. Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos.
    Cell. 1997 Aug 22;90(4):707-16 PMID: 9288750
  35. A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus.
    Genes Dev. 1997 Sep 15;11(18):2359-70 PMID: 9308964
  36. Analysis of the signaling activities of localization mutants of beta-catenin during axis specification in Xenopus.
    J Cell Biol. 1997 Oct 6;139(1):229-43 PMID: 9314542
  37. Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p.
    Mol Cell Biol. 1997 Dec;17(12):7230-6 PMID: 9372955
  38. Wnt signaling: a common theme in animal development.
    Genes Dev. 1997 Dec 15;11(24):3286-305 PMID: 9407023
  39. POP-1 and anterior-posterior fate decisions in C. elegans embryos.
    Cell. 1998 Jan 23;92(2):229-39 PMID: 9458047
  40. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
    Nature. 1998 Feb 19;391(6669):806-11 PMID: 9486653
  41. Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin.
    EMBO J. 1998 Mar 2;17(5):1371-84 PMID: 9482734
  42. Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling.
    Cell. 1998 Jul 10;94(1):109-18 PMID: 9674432
  43. Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways.
    Genes Dev. 1998 Aug 15;12(16):2610-22 PMID: 9716412
  44. Asymmetric cell division: lessons from flies and worms.
    Curr Opin Genet Dev. 1998 Aug;8(4):392-9 PMID: 9729713
  45. Differential localization of two myosins within nematode thick filaments.
    Cell. 1983 Sep;34(2):477-90 PMID: 6352051
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.15.2028
Published
1999-08-01
Pages
2028-38
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316921
Subset
IM
Grants
NIGMS NIH HHS · F32 GM016981 · United States
NIGMS NIH HHS · T32 GM007413 · United States
NIGMS NIH HHS · R01 GM049869 · United States
NICHD NIH HHS · T32 HD007348 · United States
NICHD NIH HHS · HD07348 · United States
NIGMS NIH HHS · GM16981 · United States
NIGMS NIH HHS · GM07413 · United States
Databases
GENBANK
AF159950
External Links
PubMed source DOI: 10.1101/gad.13.15.2028 PMC Full Text View journal details
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