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PMID: 8628320 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1.

Molecular and cellular biology ·Vol. 16 ·No. 5 ·1996-05-00 ·Pages 2518-26

Rubin-Bejerano I, Mandel S, Robzyk K, Kassir Y

Abstract

The transcription of meiosis-specific genes, as well as the initiation of meiosis, in the budding yeast Saccharomyces cerevisiae depends on IME1. IME1 encodes a transcriptional activator which lacks known DNA binding motifs. In this study we have determined the mode by which Ime1 specifically activates the transcription of meiotic genes. We demonstrate that Ime1 is recruited to the promoters of meiotic genes by interacting with a DNA-binding protein, Ume6. This association between Ime1 and Ume6 depends on both starvation and the activity of a protein kinase, encoded by RIM11 In the absence of Ime1, Ume6 represses the transcription of meiotic genes. However, in the presence of Ime1, or when Ume6 is fused in frame to the Gal4 activation domain, Ume6 is converted from a repressor to an activator, resulting in the transcription of meiosis-specific genes and the formation of asci.

MeSH Terms
Base Sequence Binding Sites DNA-Binding Proteins/metabolism Fungal Proteins/metabolism Gene Expression Regulation, Fungal Genes, Fungal Genotype Meiosis Models, Genetic Molecular Sequence Data Nuclear Proteins/metabolism Oligodeoxyribonucleotides Recombinant Fusion Proteins/metabolism Repressor Proteins Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins Suppression, Genetic Trans-Activators/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae IME1 protein, S cerevisiae Nuclear Proteins Oligodeoxyribonucleotides Recombinant Fusion Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors UME6 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rubin-Bejerano I
Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
Mandel S
Robzyk K
Kassir Y
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48 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.5.2518
Published
1996-05-00
Pages
2518-26
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231241
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.5.2518 PMC Full Text View journal details
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