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PMID: 2651894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Dependence of inessential late gene expression on early meiotic events in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 215 ·No. 3 ·1989-02-00 ·Pages 490-500

Kao G, Mannix DG, Holaway BL, Finn MC, Bonny AE, Clancy MJ

Abstract

SPR3 is one of at least nine genes which are expressed in sporulating Saccharomyces cerevisiae cells at the time of meiosis I. We show below that strains homozygous for null alleles of SPR3 are capable of normal meiosis and the production of viable ascospores. We have also monitored SPR3 expression in a series of strains that are defective in meiotic development, using an SPR3:lacZ fusion carried on a single copy plasmid. beta-Galactosidase activity occurred at wild-type levels in diploid strains homozygous for mutations in spo13, rad50, rad57 and cdc9, but was greatly reduced in strains carrying cdc8 or spo7 defects. We conclude that SPR3 expression is a valid monitor of early meiotic development, even though the gene is inessential for the sporulation process.

MeSH Terms
Gene Expression Regulation Genes, Fungal Meiosis Mutation Phenotype Plasmids Restriction Mapping Saccharomyces cerevisiae/genetics,physiology Spores, Fungal/genetics
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kao G
Department of Biological Sciences, University of Notre Dame, IN 46556.
Mannix D G
Holaway B L
Finn M C
Bonny A E
Clancy M J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00427048
Published
1989-02-00
Pages
490-500
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00427048
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