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PMID: 2183020 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Positive control of sporulation-specific genes by the IME1 and IME2 products in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 2104-10

Mitchell AP, Driscoll SE, Smith HE

Abstract

In the yeast Saccharomyces cerevisiae, meiosis and spore formation require the induction of sporulation-specific genes. Two genes are thought to activate the sporulation program: IME1 and IME2 (inducer of meiosis). Both genes are induced upon entry into meiosis, and IME1 is required for IME2 expression. We report here that IME1 is essential for expression of four sporulation-specific genes. In contrast, IME2 is not absolutely essential for expression of the sporulation-specific genes, but contributes to their rapid induction. Expression of IME2 from a heterologous promoter permits the expression of these sporulation-specific genes, meiotic recombination, and spore formation in the absence of IME1. We propose that the IME1 and IME2 products can each activate sporulation-specific genes independently. In addition, the IME1 product stimulates sporulation-specific gene expression indirectly through activation of IME2 expression.

MeSH Terms
Blotting, Northern DNA Mutational Analysis DNA Probes Gene Expression Genes, Fungal RNA, Fungal/genetics RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Spores, Fungal/physiology Transcription, Genetic
Chemicals
DNA Probes RNA, Fungal RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mitchell A P
Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Driscoll S E
Smith H E
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21 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.2104-2110.1990
Published
1990-05-00
Pages
2104-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360558
Subset
IM
Grants
NIGMS NIH HHS · GM3951 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.5.2104-2110.1990 PMC Full Text View journal details
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